CAJ | 학술논문

目的：探讨吲哚胺2，3-双加氧酶（indoleamine 2，3-dioxygenase，IDO）在 T 细胞介导的排斥反应移植肾组织中的表达及临床意义。方法：收集郑州大学第一附属医院2008年4月—2012年10月经病理诊断为发生 T 细胞介导的排斥反应的移植肾组织标本38例，另随机选择10例肾穿刺活组织检查正常的肾组织标本作为对照组。应用鼠抗人 IDO 单克隆抗体、按二步法进行免疫组织化学染色，检测排斥反应移植肾组织中 IDO 的表达情况。结果：正常肾组织中不表达 IDO，T 细胞介导的排斥反应移植肾组织中 IDO 的阳性表达率为73．7％，差异有统计学意义（P ＜0．01）；且 T 细胞介导的急性排斥反应移植肾组织中 IDO 阳性表达率明显高于 T 细胞介导的慢性排斥反应移植肾组织（P ＜0．05）。在 T 细胞介导的不同病理分级急性排斥反应移植肾组织中 IDO 阳性表达率差异有统计学意义（P ＜0．05）。移植肾组织中 IDO 的阳性表达强度与 T 细胞介导的急性排斥反应程度呈负相关（r＝－0．696，P ＜0．05）。结论：IDO 参与肾移植术后免疫状态的调节，高表达的 IDO 可降低 T 细胞介导的排斥反应。
목적：탐토신타알2，3-쌍가양매（indoleamine 2，3-dioxygenase，IDO）재 T 세포개도적배척반응이식신조직중적표체급림상의의。방법：수집정주대학제일부속의원2008년4월—2012년10월경병리진단위발생 T 세포개도적배척반응적이식신조직표본38례，령수궤선택10례신천자활조직검사정상적신조직표본작위대조조。응용서항인 IDO 단극륭항체、안이보법진행면역조직화학염색，검측배척반응이식신조직중 IDO 적표체정황。결과：정상신조직중불표체 IDO，T 세포개도적배척반응이식신조직중 IDO 적양성표체솔위73．7％，차이유통계학의의（P ＜0．01）；차 T 세포개도적급성배척반응이식신조직중 IDO 양성표체솔명현고우 T 세포개도적만성배척반응이식신조직（P ＜0．05）。재 T 세포개도적불동병리분급급성배척반응이식신조직중 IDO 양성표체솔차이유통계학의의（P ＜0．05）。이식신조직중 IDO 적양성표체강도여 T 세포개도적급성배척반응정도정부상관（r＝－0．696，P ＜0．05）。결론：IDO 삼여신이식술후면역상태적조절，고표체적 IDO 가강저 T 세포개도적배척반응。
Objective:To explore the expression of indoleamine 2,3-dioxygenase (IDO)in the tissue of transplanted kidney un-der T cell-mediated rejection.Methods:A total of 38 cases of tissue specimens of transplanted kidney under T cell-mediated re-jection according to pathological diagnosis from Apr 2008 to Oct 2012 in the First Affiliated Hospital of Zhengzhou University were collected.In addition,10 cases of normal renal biopsy specimens were randomly selected as control group.Mouse anti-hu-man IDO monoclonal antibody with two-step immunohistochemical staining was applied for the detection of IDO expression in the tissue of transplanted kidney under rejection.Results:There was no IDO expression in the normal renal biopsy tissue.Posi-tive expression rate of IDO was 73.7% in the tissue of transplanted kidney under T cell-mediated rejection.There was statisti-cally significant difference(P <0.01).Positive expression rate of IDO in the tissue of transplanted kidney under T cell mediated acute rejection was obviously higher than that under T cell-mediated chronic rejection(P <0.05).The differences in positive ex-pression rate of IDO in transplanted kidney tissues under different pathological grades of T cell-mediated rejection were statisti-cally significant(P <0.05).The expression intensity of IDO was negatively correlated with the grade of T cell-mediated acute rejection(r=-0.696,P <0.05).Conclusions:IDO is involved in the immune regulation after renal transplantation.Higher ex-pression of IDO can significantly reduce T cell-mediated acute rejection.