CAJ | 학술논문

以亮叶桦（Betula luminifera）4个优良无性系为材料，通过1/2 MS、MS、B5、MS?B5四种基本培养基和分段扦插的方法筛选亮叶桦微扦插的最佳培养基以及外植体的最适茎段部位。结果表明：亮叶桦株高、根数、根长性状在不同培养基间存在极显著或显著差异，多重比较表明株高和根数表现最佳培养基为MS?B5，根长性状表现最佳培养基为 B5。以不同部位的茎段为外植体，4个无性系在 MS?B5培养基中微扦插，其株高和根数性状存在极显著差异，叶宽生长存在显著差异，而根长和叶长无显著差异，以株高生长为选择指标，最佳外植体为中下部茎段，以根数为选择指标，中上部茎段作为外植体优于下部茎段。综合分析表明，以中部茎段为外植体，选用MS?B5培养基，可适用于亮叶桦不同无性系的微扦插快繁。
이량협화（Betula luminifera）4개우량무성계위재료，통과1/2 MS、MS、B5、MS?B5사충기본배양기화분단천삽적방법사선량협화미천삽적최가배양기이급외식체적최괄경단부위。결과표명：량협화주고、근수、근장성상재불동배양기간존재겁현저혹현저차이，다중비교표명주고화근수표현최가배양기위MS?B5，근장성상표현최가배양기위 B5。이불동부위적경단위외식체，4개무성계재 MS?B5배양기중미천삽，기주고화근수성상존재겁현저차이，협관생장존재현저차이，이근장화협장무현저차이，이주고생장위선택지표，최가외식체위중하부경단，이근수위선택지표，중상부경단작위외식체우우하부경단。종합분석표명，이중부경단위외식체，선용MS?B5배양기，가괄용우량협화불동무성계적미천삽쾌번。
Four different clones of Betula luminifera were selected for experiment of microcutting propagation in four types of medium with different stem segments. The results showed that the height, rooting numbers and root length of cuttings had significant differences among different mediums. Multiple comparisons showed that the optimal medium was MS?B5 for height growth and rooting numbers, and B5 for root length. Different stem segment of four clones in MS?B5 had significant differences of height growth, rooting numbers, and leaf width, except root length and leaf length. The experiments indicated that the explants from lower stem segments had better height growth, while the upper stem segments had more rooting numbers.