中国水产科学
中國水產科學
중국수산과학
Journal of Fishery Sciences of China
2014年
6期
1253-1259
,共7页
张林宝%孙伟%蔡文贵%贾晓平
張林寶%孫偉%蔡文貴%賈曉平
장림보%손위%채문귀%가효평
菲律宾蛤仔%谷氧还蛋白%微生物侵染%Cd%基因表达
菲律賓蛤仔%穀氧還蛋白%微生物侵染%Cd%基因錶達
비률빈합자%곡양환단백%미생물침염%Cd%기인표체
Venerupis philippinarum%glutaredoxin%bacterial challenge%Cd%gene expression
研究了菲律宾蛤仔(Venerupis philippinarum)两种谷氧还蛋白VpGrx1和VpGrx2 mRNA在鳗弧菌(Vibrio an-guillarum)、藤黄微球菌(Micrococcus luteus)和重金属镉(Cd)作用下的表达调控规律。结果表明,正常菲律宾蛤仔VpGrx1和VpGrx2在所检测组织中均有表达,其中VpGrx1在鳃中的表达量最高,而VpGrx2在蛤仔血淋巴细胞、鳃和肝胰腺中的表达量均较高。鳗弧菌和藤黄微球菌侵染在某些时段可显著诱导VpGrx1和VpGrx2基因的表达水平(P<0.05)。另外,0.8μg/L、8μg/L和80μg/L Cd处理30 d后,菲律宾蛤仔肝胰腺中VpGrx1和VpGrx2基因转录水平显著升高(P<0.05),血淋巴细胞中 VpGrx1基因表达被显著抑制(P<0.05)而 VpGrx2基因表达被显著诱导(P<0.05)。以上结果表明, VpGrx1和VpGrx2在菲律宾蛤仔的固有免疫反应和抗氧化胁迫中发挥着重要作用。
研究瞭菲律賓蛤仔(Venerupis philippinarum)兩種穀氧還蛋白VpGrx1和VpGrx2 mRNA在鰻弧菌(Vibrio an-guillarum)、籐黃微毬菌(Micrococcus luteus)和重金屬鎘(Cd)作用下的錶達調控規律。結果錶明,正常菲律賓蛤仔VpGrx1和VpGrx2在所檢測組織中均有錶達,其中VpGrx1在鰓中的錶達量最高,而VpGrx2在蛤仔血淋巴細胞、鰓和肝胰腺中的錶達量均較高。鰻弧菌和籐黃微毬菌侵染在某些時段可顯著誘導VpGrx1和VpGrx2基因的錶達水平(P<0.05)。另外,0.8μg/L、8μg/L和80μg/L Cd處理30 d後,菲律賓蛤仔肝胰腺中VpGrx1和VpGrx2基因轉錄水平顯著升高(P<0.05),血淋巴細胞中 VpGrx1基因錶達被顯著抑製(P<0.05)而 VpGrx2基因錶達被顯著誘導(P<0.05)。以上結果錶明, VpGrx1和VpGrx2在菲律賓蛤仔的固有免疫反應和抗氧化脅迫中髮揮著重要作用。
연구료비률빈합자(Venerupis philippinarum)량충곡양환단백VpGrx1화VpGrx2 mRNA재만호균(Vibrio an-guillarum)、등황미구균(Micrococcus luteus)화중금속력(Cd)작용하적표체조공규률。결과표명,정상비률빈합자VpGrx1화VpGrx2재소검측조직중균유표체,기중VpGrx1재새중적표체량최고,이VpGrx2재합자혈림파세포、새화간이선중적표체량균교고。만호균화등황미구균침염재모사시단가현저유도VpGrx1화VpGrx2기인적표체수평(P<0.05)。령외,0.8μg/L、8μg/L화80μg/L Cd처리30 d후,비률빈합자간이선중VpGrx1화VpGrx2기인전록수평현저승고(P<0.05),혈림파세포중 VpGrx1기인표체피현저억제(P<0.05)이 VpGrx2기인표체피현저유도(P<0.05)。이상결과표명, VpGrx1화VpGrx2재비률빈합자적고유면역반응화항양화협박중발휘착중요작용。
Glutaredoxins (Grxs) are ubiquitous small disulfide oxidoreductases that play a crucial role in response to oxidative stress and regulation of growth and development. However, the structural features and function of Grxs in marine invertebrates are still unknown compared with those of Escherichia coli, yeast, mammals, and plants. In this study, quantitative RT-PCR was performed to assay the temporal expression patterns of VpGrx1 and VpGrx2 post Vibrio anguillarum, Micrococcus luteus and Cd challenge. VpGrx1 and VpGrx2 transcripts could be detected in all the ex-amined tissues, including gills, hepatopancreas, adductor muscles, mantle, and hemocytes. VpGrx1 was found to be most abundantly expressed in gills, while VpGrx2 was highly expressed in haemocytes, hepatopancreas and gills, which is consistent with the viewpoint that the hepatopancreas and gills are the most important organs in the host immune de-fense and detoxification systems. Bacterial challenging significantly up-regulated mRNA expression of VpGrx1 and VpGrx2at different time intervals, with a maximum 2.96 fold rise. The increased VpGrx1 and VpGrx2 transcripts may be associated with increasing reactive oxygen species (ROS) that is generated after apathogen challenge. In addition, the expression levels of VpGrx1 and VpGrx2 in the hepatopancreas of Venerupi sphilippinarum were also significantly up-regulated by 0.8, 8, and 80μg/L Cd with a larger amplitude in VpGrx2, and the results showed an obviously concen-tration-dependent increase. The highest expression level for VpGrx2 (34.87 fold) in hepatopancreas was observed after 80μg/L Cd exposure for 30 days. More or less compatible transcriptional modulation patterns were also experienced in several organisms, such as Pisum sativum, Glomus intraradices, and Homo sapiens. It has been demonstrated that Cd can induce oxidative stress by increasing levels of ROS. The increase in VpGrx1 and VpGrx2 expression in the hepato-pancreas of V. philippinarum could be induced by the production of ROS generated by Cd, suggesting aGrx protective role in the defense against oxidative stress. With regard to hemocytes, the expression of VpGrx1 mRNA decreased sharply (0.03 fold at 0.8 and 80μg/L Cd, 0.16 fold at 8μg/L Cd), while the expression levels of VpGrx2 were increased significantly (4.77 fold at 0.8μg/L Cd, 4.05 fold at 8μg/L Cd, and 4.63 fold at 80μg/L Cd) after 30 d. The selective up-or down-regulation of VpGrxs gene expression highlighted the fact that these isoenzymes probably played divergent physiological roles during the detoxification of Cd in hemocytes of V. philippinarum. Future studies should explore the diverse expression patterns of VpGrx1 and VpGrx2 genes in hemocytes of V. philippinarum after exposure to Cd. In conclusion, all these results suggested that VpGrx1 and VpGrx2 may be important proteins involved in host immune defense and heavy metal detoxification.
