CAJ | 학술논문

以国产小型蝰科毒蛇短尾蝮（Gloydius brevicaudus）、岩栖蝮（Gloydius saxatilis）、乌苏里蝮（Gloydius ussuriensis）、山烙铁头（Ovophis monticola）、原矛头蝮（Protobothrops mucrosquamatus）和竹叶青（Trimeresurus stejnegeri）蛇毒为对象，用国产商用抗蝮蛇毒血清和抗五步蛇毒血清分别检测这6种蛇毒的免疫原性。间接ELISA和western blot两种方法的检测结果表明：6种蛇毒有共同的抗原组分，且在免疫反应中大部分组分表现出免疫原性；同种蛇毒和抗血清之间显示出最高的免疫结合强度；近缘物种间的免疫结合强度更接近；随着抗体浓度的升高，免疫结合强度增加。抗蝮蛇毒血清对短尾蝮、乌苏里蝮和岩栖蝮蛇毒的免疫中和效价高于抗五步蛇毒血清；两种抗蛇毒血清中和山烙铁头、原矛头蝮和竹叶青蛇毒的效价相近。
이국산소형호과독사단미복（Gloydius brevicaudus）、암서복（Gloydius saxatilis）、오소리복（Gloydius ussuriensis）、산락철두（Ovophis monticola）、원모두복（Protobothrops mucrosquamatus）화죽협청（Trimeresurus stejnegeri）사독위대상，용국산상용항복사독혈청화항오보사독혈청분별검측저6충사독적면역원성。간접ELISA화western blot량충방법적검측결과표명：6충사독유공동적항원조분，차재면역반응중대부분조분표현출면역원성；동충사독화항혈청지간현시출최고적면역결합강도；근연물충간적면역결합강도경접근；수착항체농도적승고，면역결합강도증가。항복사독혈청대단미복、오소리복화암서복사독적면역중화효개고우항오보사독혈청；량충항사독혈청중화산락철두、원모두복화죽협청사독적효개상근。
The immunogenicity of venoms from six small domestic species of Viperidae venomous snakes (Gloydius brevicaudus , Gloydius saxatilis , Gloydius ussuriensis , Ovophis monticola , Protobothrops mucrosquamatus and Trimeresurus stejnegeri ) is investigated in this study . The immunological cross‐reactions between venoms and two commercial antivenoms (G .brevicaudus antivenom and D .acutus antivenom) are evaluated by ELISA and western blot . The results show that venoms from these six snakes are shared many common antigenic components ,and most venom components express antigenicity . Homologous venom and antivenom express the highest immunoreactivity . The immunoreactivity of closely related species is more similar . Along with the rising of antivenom concentration , the immunoreactivity increases .The G .brevicaudus antivenom is more effective in neutralizing the venoms of G .brevicaudus , G .saxatilis and G .ussuriensis than D .acutus antivenom .Neutralization titer of these two commercial antivenoms against O .monticola ,P .mucrosquamatus and T .stejnegeri is similar .