CAJ | 학술논문

采用包埋法，以CMC-膨润土混合凝胶为载体，将从活性污泥中驯化分离出的高效菌株固定，制备固定化颗粒，应用于吸附降解萘的过程。研究结果表明，固定化颗粒吸附降解萘的机制包括4个过程，即：吸附-脱附-再吸附-生物降解，同时这4个过程与固定化颗粒内部的细菌生长繁殖呈现对应关系。以6%的接种量（V/V水溶胶）且未经去营养化处理制备得到的固定化颗粒降解萘的效果最佳，在30℃下，32 h内将初始质量浓度为40 mg/L的萘液降解至检测不到的水平。对固定化颗粒进行反复实验，结果显示该固定化颗粒不仅机械强度较好，在130 r/min的条件下连续运行20 d不破碎，而且在反复实验中的耐受性也大大增强，一直保持较高的生物活性，萘的降解率维持在100%，基本不变。
채용포매법，이CMC-팽윤토혼합응효위재체，장종활성오니중순화분리출적고효균주고정，제비고정화과립，응용우흡부강해내적과정。연구결과표명，고정화과립흡부강해내적궤제포괄4개과정，즉：흡부-탈부-재흡부-생물강해，동시저4개과정여고정화과립내부적세균생장번식정현대응관계。이6%적접충량（V/V수용효）차미경거영양화처리제비득도적고정화과립강해내적효과최가，재30℃하，32 h내장초시질량농도위40 mg/L적내액강해지검측불도적수평。대고정화과립진행반복실험，결과현시해고정화과립불부궤계강도교호，재130 r/min적조건하련속운행20 d불파쇄，이차재반복실험중적내수성야대대증강，일직보지교고적생물활성，내적강해솔유지재100%，기본불변。
A strain was isolated from the activated sludge to degrade naphthalene effectively. Bentonite and carboxyl-methyl cellulose (CMC) were selected as the carriers to immobilize the strain. The process of the immobilized beads degrading naphthalene was investigated. The results showed that the immobilized beads degrading naphthalene consisted of four steps: adsorption - desorption - re-adsorption - biodegradation, and there was a corresponding relation between the four steps and the growth of bacteria in the immobilized beads. The optimum experimental conditions were of 6% (V/Vaqueous sol) suspension without the nutritional treatment, 40 mg/L of the initial naphthalene concentration and 30℃. Naphthalene was degraded to the undetectable level in 32 h. The reuse of the immobilized beads allows us to get 10 cycles. During the reusing process, the tolerance and biological activity of the immobilized beads were greatly enhanced, and the naphthalene degradation rate was almost remained unchanged at 100%.