CAJ | 학술논문

目的：探讨过氧化物酶增殖物激活受体-β（peroxisome proliferater activated receptors-beta，PPAR-β）对脊髓损伤（spinal cord injury，SCI）大鼠脊髓组织及运动功能的影响。方法45只SD大鼠随机分为假手术组（Sham组）、脊髓损伤组（SCI组）和PPAR-β受体激动剂GWO742治疗组（GWO742组）。Sham组大鼠仅做椎板切除术不损伤脊髓组织，GWO742组和SCI组大鼠分别采用改良Allen`s打击法制作SCI模型，并于术后1h开始分别通过腹腔注射同等剂量0.3mg/（kg·d）的PPAR-β受体激动剂GWO742和生理盐水。各组大鼠分别于术后1、3、7、14、21d随机抽取大鼠行BBB评分；术后24h随机抽取大鼠10只，处死取脊髓组织行HE染色观察脊髓组织病理变化，TUNEL法染色检测细胞凋亡。结果 BBB评分结果显示，术后3、7、14、21d，GWO742组大鼠较SCI组大鼠BBB评分高，且差异均有统计学意义（P<0.05）；HE染色结果显示SCI组大鼠脊髓组织充血、水肿、损伤中心区出现液化坏死并伴有炎性细胞浸润，GWO742组大鼠脊髓出血、坏死范围及炎性细胞浸润明显轻于SCI组；TUNEL染色显示伤后24h，SCI组和GWO742组损伤脊髓组织均可发现TUNEL阳性细胞，而GWO742组TUNEL阳性细胞数明显少于SCI组（P<0.05）。结论 PPAR-β受体激活后能够减轻SCI大鼠脊髓组织充血、水肿等病理学改变，抑制神经元凋亡，促进大鼠后肢运动功能恢复。
목적：탐토과양화물매증식물격활수체-β（peroxisome proliferater activated receptors-beta，PPAR-β）대척수손상（spinal cord injury，SCI）대서척수조직급운동공능적영향。방법45지SD대서수궤분위가수술조（Sham조）、척수손상조（SCI조）화PPAR-β수체격동제GWO742치료조（GWO742조）。Sham조대서부주추판절제술불손상척수조직，GWO742조화SCI조대서분별채용개량Allen`s타격법제작SCI모형，병우술후1h개시분별통과복강주사동등제량0.3mg/（kg·d）적PPAR-β수체격동제GWO742화생리염수。각조대서분별우술후1、3、7、14、21d수궤추취대서행BBB평분；술후24h수궤추취대서10지，처사취척수조직행HE염색관찰척수조직병리변화，TUNEL법염색검측세포조망。결과 BBB평분결과현시，술후3、7、14、21d，GWO742조대서교SCI조대서BBB평분고，차차이균유통계학의의（P<0.05）；HE염색결과현시SCI조대서척수조직충혈、수종、손상중심구출현액화배사병반유염성세포침윤，GWO742조대서척수출혈、배사범위급염성세포침윤명현경우SCI조；TUNEL염색현시상후24h，SCI조화GWO742조손상척수조직균가발현TUNEL양성세포，이GWO742조TUNEL양성세포수명현소우SCI조（P<0.05）。결론 PPAR-β수체격활후능구감경SCI대서척수조직충혈、수종등병이학개변，억제신경원조망，촉진대서후지운동공능회복。
Object To investigate the effect of PPAR-βon spinal cord and hind limb motor function in rats with spinal cord injury(SCI). Method 45 SD rats were randomly divided into 3 groups of sham(sham operated),SCI(SCI model ) and GWO742(treated with PPAR-βagonist GWO742).The rats in the Sham group were only performed laminectomy without spinal cord contusion. In group SCI and GWO742,SCI model was estalbished by a modified Allen`s method.1 hour after operation,rats in group GWO742 and SCI were administered GWO742(0.3 mg/kg i.p. bolus) and saline(0.3mg/kg i.p. bolus) respectively once a day. The spinal cord function of rats were evaluated using BBB scales at 1,3,7,14,21 day past injury;Rats were sacrificed at 24h after operation. The 1esion areas of the spinal cord were dissected for morphological studies by hematoxylin and eosin staining. Cell apoptosis was examined using the Terminal deoxynucleotidyl transferase dUTP nick end labeling(TUNEL) assay. Result Behavioral score of the GWO742 group was significantly higher than that of SCI group at 3,7,14,21d post-injury(P<0.05);HE stain showed bleeding,hydroncus,liquefaction necrosis combined with inflammatory cells infiltrating in SCI group. In group GWO742,there was less degree of bleeding,hydrops and less scope of neuro-necrosis and inflammatory reaction. The apoptosis cells positive for TUNEL were detectable in group SCI and GWO742. In group GWO742,the number of apoptotic cells were decreased as compared with those in group SCI(P<0.05).Conclusions The activation of PPAR-βreceptor can reduce SCI rats spinal cord tissue congestion,edema and other pathological changes,inhibit neuronal apoptosis,promote hindlimb motor function recovery.