CAJ | 학술논문

目的：通过测定肺癌患者血小板源性CD40、CD40配体（CD40L）、CD62P的表达水平，探讨血小板源性CD40/CD40L在肺癌外周血中的表达及其临床意义。方法体外分离肺癌患者的血小板，用二磷酸腺苷（ADP）体外诱导前后采用流式细胞仪对其表面表达的CD40、CD40L、CD62P进行测定，并与健康人作比较。结果（1）ADP诱导前，肺癌组血小板源性CD40、CD40L、CD62P表达水平明显高于对照组（P均<0.01）。ADP诱导后，肺癌组血小板源性CD40、CD62P表达水平明显高于对照组（P均<0.01）（2）ADP诱导前后，不同病理类型肺癌患者血小板源性CD40、CD40L、CD62P表达水平的差异均无统计学意义（P均>0.05）。（3）ADP诱导前后，有远处转移患者血小板源性CD40L、CD62P表达水平明显高于无远处转移患者（P<0.05）。结论（1）肺癌患者血小板源性CD62P、CD40L表达水平增加，且表达水平与肺癌的进展程度相关，血小板源性CD40/CD40L系统在肺癌的发病中具有重要作用。（2）检测血小板源性CD40L、CD62P表达水平对研究肺癌的临床分期、预后判断有一定的参考价值。
목적：통과측정폐암환자혈소판원성CD40、CD40배체（CD40L）、CD62P적표체수평，탐토혈소판원성CD40/CD40L재폐암외주혈중적표체급기림상의의。방법체외분리폐암환자적혈소판，용이린산선감（ADP）체외유도전후채용류식세포의대기표면표체적CD40、CD40L、CD62P진행측정，병여건강인작비교。결과（1）ADP유도전，폐암조혈소판원성CD40、CD40L、CD62P표체수평명현고우대조조（P균<0.01）。ADP유도후，폐암조혈소판원성CD40、CD62P표체수평명현고우대조조（P균<0.01）（2）ADP유도전후，불동병리류형폐암환자혈소판원성CD40、CD40L、CD62P표체수평적차이균무통계학의의（P균>0.05）。（3）ADP유도전후，유원처전이환자혈소판원성CD40L、CD62P표체수평명현고우무원처전이환자（P<0.05）。결론（1）폐암환자혈소판원성CD62P、CD40L표체수평증가，차표체수평여폐암적진전정도상관，혈소판원성CD40/CD40L계통재폐암적발병중구유중요작용。（2）검측혈소판원성CD40L、CD62P표체수평대연구폐암적림상분기、예후판단유일정적삼고개치。
Objective To investigate the expressions of platelet-derived CD40、CD40 ligand(CD40L) in patients with lung cancer,and to evaluate the clinical signif icance of platelet-derived CD40/CD40L in lung cancer.Methods:Platelets were isolated from patients with lung cancer and normal subjects.The expression rates of platelet-derived CD40、CD40L、CD62P were measured by flow cytometry before and after adenosine diphosphate(ADP) inducing in vitro. Results (1) The expression of platelet-derived CD40、CD40L、CD62P were significantly increased in patients with lung cancer as compared with normal volunteers before induced by ADP(P<0.01).After induced by ADP,the expression of CD40、CD62P in patients were significantly higher than those of normal volunteers(P<0.01).(2) Before and after induced by ADP,the expression of platelet-derived CD40、CD40L、CD62P showed no statistically significant difference among all pathological types of patients with lung cancer(P>0.05).(3) Before and after induced by ADP,The expression of platelet-derived CD40L and CD62P were higher in the patients with metastasis than those of without metastasis(P<0.05).Conclusions (1)These results suggested that there were high levels expression of platelet-derived CD40L、CD62P in patients with lung cancer.Therefore the levels of them were correlated with the stages of lung cancer.The system of platelet-derived CD40/CD40L might have a potent effect in pathogenesis of lung cancer.(2) Measurement of platelet-derived CD40L、CD62P may therefore provide a tool for monitoring the clinical course of lung cancer.