CAJ | 학술논문

为了探讨氧化苦参碱-黄芪多糖中草药复方对抗新城疫病毒（NDV）感染效果，旨在为筛选确实有效的防治鸡新城疫的中药组方，将14日龄非免疫健康罗曼公雏鸡150羽随机均分为5组，1组～3组分别为氧化苦参碱-黄芪多糖复方高、中、低剂量组，4组为免疫对照组，5组为空白对照组。除空白对照组外均用新城疫Ⅳ系疫苗滴鼻、点眼免疫，28日龄重复免疫。在首次免疫的同时，1组～3组分别口服高、中、低剂量氧化苦参碱-黄芪多糖复方药物，疫苗对照组和空白对照组口服等量生理盐水，每天1次，连续3 d。分别于14、28和42日龄采血，用双抗夹心 ELISA 法检测血清中 IL-2和 IFN-γ的浓度。结果显示，复方的高、中、低剂量在各时间点血清 IL-2浓度显著高于免疫对照组（P ＜0．05）；血清 IFN-γ浓度均显著高于免疫对照组（P＜0．05）。说明中药成分复方能显著增强雏鸡对新城疫疫苗的免疫应答，高、中剂量的效果较好，可用于促进细胞免疫，提高机体抗病能力。
위료탐토양화고삼감-황기다당중초약복방대항신성역병독（NDV）감염효과，지재위사선학실유효적방치계신성역적중약조방，장14일령비면역건강라만공추계150우수궤균분위5조，1조～3조분별위양화고삼감-황기다당복방고、중、저제량조，4조위면역대조조，5조위공백대조조。제공백대조조외균용신성역Ⅳ계역묘적비、점안면역，28일령중복면역。재수차면역적동시，1조～3조분별구복고、중、저제량양화고삼감-황기다당복방약물，역묘대조조화공백대조조구복등량생리염수，매천1차，련속3 d。분별우14、28화42일령채혈，용쌍항협심 ELISA 법검측혈청중 IL-2화 IFN-γ적농도。결과현시，복방적고、중、저제량재각시간점혈청 IL-2농도현저고우면역대조조（P ＜0．05）；혈청 IFN-γ농도균현저고우면역대조조（P＜0．05）。설명중약성분복방능현저증강추계대신성역역묘적면역응답，고、중제량적효과교호，가용우촉진세포면역，제고궤체항병능력。
In order to evaluate the efficacy of oxymatrine-Astragalus polysaccharide(OTM-APS)against Newcastle disease virus (NDV)infection,The prevention efficacy of compound on Newcastle disease was evaluated.One hundred and fifty 14 days old Roman cocks without vaccination were randomly divided into 5 groups.the chickens in groups 1,2 and 3 were first intranasally administrated by NDV-Ⅳ vaccine.At the same time,the cocks in groups 1,2 and 3 were orally administrated with OTM-APS compounds of high,middle and low doses,respectively.Group 4 was vaccine control and Group 5 was blank control. Cocks in group 4 was vaccinated by vaccine and taken physiological saline,once a day for three days.The cocks were boost vaccinated at 28 days old.The concentrations of IL-2 and IFN-γin the sera of cocks in the 5 groups were detected by double antibody sandwich ELISA at 14,28 and 42 days old.The results showed that the concentrations of IL-2 and IFN-γin sera of the cocks treated with medicine were significantly high-er than that of the cocks in the vaccine control group (P <0.05).It indicated that OTM-APS compound could significantly strengthen the immune response of chickens to Newcastle disease vaccine and enhance the resistance against the disease..