CAJ | 학술논문

为比较不同核酸提取方法检测禽流感病毒敏感性的差异，将 H5N1禽流感抗原做10－2～10－10稀释，分别用 Trizol 法、磁珠法和膜吸附法提取上述抗原稀释度核酸模板，采用荧光 RT-PCR 对其进行评价。结果表明，在不同稀释度的抗原的检测中，3种核酸提取方法以膜吸附法提取效率最高，其依次为磁珠法、Trizol 法，经方差分析，3种核酸提取方法之间存在显著性差异（P＜0．05）。说明 3种核酸提取方法提取效率存在显著差异，核酸提取效率的高低影响禽流感病毒荧光 RT-PCR 检测的敏感性，建议实验室根据实际情况选择核酸提取的方法。
위비교불동핵산제취방법검측금류감병독민감성적차이，장 H5N1금류감항원주10－2～10－10희석，분별용 Trizol 법、자주법화막흡부법제취상술항원희석도핵산모판，채용형광 RT-PCR 대기진행평개。결과표명，재불동희석도적항원적검측중，3충핵산제취방법이막흡부법제취효솔최고，기의차위자주법、Trizol 법，경방차분석，3충핵산제취방법지간존재현저성차이（P＜0．05）。설명 3충핵산제취방법제취효솔존재현저차이，핵산제취효솔적고저영향금류감병독형광 RT-PCR 검측적민감성，건의실험실근거실제정황선택핵산제취적방법。
To compare the efficiency of different nucleic acid extraction methods for detection of avian influ-enza virus.H5N1 avian influenza virus was used as template,Trizol,magnetic beads and membrane ad-sorption methods were used for nucleic acid extraction,H5N1 avian influenza virus were diluted from 10 -2 to 10-10 ,the efficiency of the three methods were evaluated through real-time RT-PCR.The variance anal-ysis showed there were significance differences among the three different methods(P<0.05).The mem-brane adsorption extraction method is the most efficient one,and followed by magnetic beads and Trizol extraction.The nucleic acid extraction efficiency is closely related to the sensitivity of the real-time RT-PCR results.The results suggested that the appropriate detection methods should be chosen for detection of clinical specimens based on actual situation.