CAJ | 학술논문

为探索 H9N2 AIV感染蛋鸡致输卵管功能异常的机理，对蛋鸡感染 H9N2亚型 AIV后输卵管不同部位病毒载量与病理变化进行检测与观察。以 H9N2亚型 AIV 人工感染非免疫蛋鸡，在感染后3、5、7 d 分别采取鸡输卵管组织，real-time PCR 检测感染鸡输卵管组织中 AIV 病毒载量，同时 HE 染色后观察病理组织学变化。结果表明，real-time PCR 从鸡输卵管膨大部、峡部、子宫部和阴道部均检出了禽流感病毒，其中以膨大部和子宫部病毒载量最高，分别达5．27×104拷贝／μL±3．55×103拷贝／μL 和5．52×10 4拷贝／μL±3．14×103拷贝／μL。组织病理学观察发现蛋鸡感染 H9N2亚型 AIV 后输卵管膨大部、峡部和子宫部病变明显。观察到上皮细胞脱落坏死，炎性细胞浸润腺体，腺体间隙变大甚至溶解，腺体组织充血出血。本研究证实蛋鸡感染 H9N2亚型 AIV 后输卵管组织中 AIV 病毒载量与其病理变化存在明显的正相关。
위탐색 H9N2 AIV감염단계치수란관공능이상적궤리，대단계감염 H9N2아형 AIV후수란관불동부위병독재량여병리변화진행검측여관찰。이 H9N2아형 AIV 인공감염비면역단계，재감염후3、5、7 d 분별채취계수란관조직，real-time PCR 검측감염계수란관조직중 AIV 병독재량，동시 HE 염색후관찰병리조직학변화。결과표명，real-time PCR 종계수란관팽대부、협부、자궁부화음도부균검출료금류감병독，기중이팽대부화자궁부병독재량최고，분별체5．27×104고패／μL±3．55×103고패／μL 화5．52×10 4고패／μL±3．14×103고패／μL。조직병이학관찰발현단계감염 H9N2아형 AIV 후수란관팽대부、협부화자궁부병변명현。관찰도상피세포탈락배사，염성세포침윤선체，선체간극변대심지용해，선체조직충혈출혈。본연구증실단계감염 H9N2아형 AIV 후수란관조직중 AIV 병독재량여기병리변화존재명현적정상관。
In order to explore the mechanism about the abnormal function of hens oviduct,the viral loads were detected and histologic changes were observed in hens oviduct infected with H9N2 AIV.Hens were infected with H9N2 AIV experimentally.Tissue samples of the hen oviducts were collected at 3,5,and 7 days post-inoculation.real-time PCR was used to detect the AIV viral loads and HE staining was employed to observe the histopathological lesions in the hen oviducts.The results showed that the AIV viral loads in hen oviducts were detected.The viral loads in magnum and uterus were higher,5.27× 10 4 ±3.55 × 10 3 copies/μL and 5.52×104 ±3.14×103 copies/μL respectively.Obvious pathological lesions were presented in magnum,isthmus and uterus.Epithelial villus fell off,broad hemorrhage,inflammatory cell invasion and gland necrosis were observed in hen oviducts.This study showed that the viral loads were positively correlated with the histopathological lesions in hen oviducts.