CAJ | 학술논문

采用稀盐酸溶剂和超声提取的组合方法从中药常山中分离常山碱，用紫外-可见分光光度计测定常山碱含量；以浸膏重量和常山碱含量作为评价指标，应用正交设计法考察提取温度、料液比和提取时间三个因素对常山碱提取率的影响。结果表明，常山碱超声提取最佳工艺：提取温度50℃，料液比1"5，提取时间1 h；在此条件下，浸膏重量为0．513 g，其中常山碱含量为0．453％，同比常山饮片中常山碱含量提高了40倍以上；三个因素对常山碱提取率的影响：提取温度＞料液比＞提取时间。说明常山碱采用超声提取工艺切实可行，具有省时、操作简便和提取率高等优点。
채용희염산용제화초성제취적조합방법종중약상산중분리상산감，용자외-가견분광광도계측정상산감함량；이침고중량화상산감함량작위평개지표，응용정교설계법고찰제취온도、료액비화제취시간삼개인소대상산감제취솔적영향。결과표명，상산감초성제취최가공예：제취온도50℃，료액비1"5，제취시간1 h；재차조건하，침고중량위0．513 g，기중상산감함량위0．453％，동비상산음편중상산감함량제고료40배이상；삼개인소대상산감제취솔적영향：제취온도＞료액비＞제취시간。설명상산감채용초성제취공예절실가행，구유성시、조작간편화제취솔고등우점。
Febrifugine from Dichroa febrifuga were separated by dilute hydrochloric acid and ultrasonic ex-traction method;extract weight and febrifugine content as evaluation index,extraction process by orthog-onal design were investigated including extraction temperature,solid-liquid ratio and extraction time;feb-rifugine content was determined by ultraviolet-visble spectrophotometer.The results showed that the opti-mial technology was as followings:50℃ extraction temperature,1 " 5 solid-liquid ratio,1 h extraction time;under above conditions,extract weight and febrifugine content were respectively up to 0.513 g and 0.453%,increasing 40 times compared to radix dichroa;the effect of three factors on the extraction rate:extraction temperature>solid-liquid ratio>extraction time.The results suggested that febrifugine extrac-tion is feasible by ultrasonic technology,which has advantages of time-saving,energy conservation and higher extraction rate.