CAJ | 학술논문

为探讨牙鲆致病性迟钝爱德华菌毒力基因携带与分布，以毒力基因为分子靶标研究其致病机理及建立快速检测方法，根据 GenBank 上的基因序列，设计2对引物扩增致病性迟钝爱德华菌毒力基因 fimA和小肠结肠炎耶尔森菌毒力基因 irp2，结果在7个供试牙鲆病例的130株致病性迟钝爱德华菌中，irp2毒力基因的阳性率为46．15％（60／130），fimA 毒力基因的阳性率为100％，且均与已发表的参考菌株相应序列高度同源，同源性分别为97．32％和97．59％。可见耶尔森菌强毒力岛（HPI）在牙鲆致病性迟钝爱德华菌中广泛分布，但不同病例分离菌株间存在差异；fimA 毒力基因存在于所有的被检致病性迟钝爱德华菌中，可以作为快速检测致病性迟钝爱德华菌的标志物。
위탐토아평치병성지둔애덕화균독력기인휴대여분포，이독력기인위분자파표연구기치병궤리급건립쾌속검측방법，근거 GenBank 상적기인서렬，설계2대인물확증치병성지둔애덕화균독력기인 fimA화소장결장염야이삼균독력기인 irp2，결과재7개공시아평병례적130주치병성지둔애덕화균중，irp2독력기인적양성솔위46．15％（60／130），fimA 독력기인적양성솔위100％，차균여이발표적삼고균주상응서렬고도동원，동원성분별위97．32％화97．59％。가견야이삼균강독력도（HPI）재아평치병성지둔애덕화균중엄범분포，단불동병례분리균주간존재차이；fimA 독력기인존재우소유적피검치병성지둔애덕화균중，가이작위쾌속검측치병성지둔애덕화균적표지물。
To investigate the carrying and distribution of the virulence genes in flounder paralichthys oliva-ceus,to study its pathogenesis and establish a rapid detection method as molecular targets,the pathogenic Edwardsiella tarda virulence genes fimA and theYersinia virulence genes irp2 were amplified,using the two pairs of primers designed according to GenBank gene sequences published.The results showed that the positive rate of irp2 gene was 46.15% (60/130),the positive rate of fimA was 100%,in 130 Edwardsiella tarda strains of the 7 tested cases,and there were high homology with the sequences of corresponding ref-erence strains in the GenBank.The homologies were 97.32% and 97.59% respectively.TheYersinia HPI was widely distributed in pathogenic Edwardsiella tarda ,but there were differences among different strains isolated from the different cases.The virulence gene fimA was detected in all pathogenic Edwards-iella tarda ,and it can be used as rapid detection markers to detect the pathogenic Edwardsiella tarda .