CAJ | 학술논문

为了探究松材线虫的耐寒性，以采自湖北省宜昌市夷陵区的松材线虫（ Bursaphelenchus xylophilus）为试验对象，研究低温及低温驯化对松材线虫存活情况的影响。结果表明：松材线虫有较强的耐低温能力，在10、5、0、－5、－10℃低温条件下，随着处理时间的延长，松材线虫存活率逐渐降低，各温度半致死时间分别为20．17、35．63、30．65、1．17 d，其中－10℃低温条件下短暂暴露，线虫全部死亡。此外，在0℃和5℃条件下经不同时间驯化后，－5℃暴露处理24 h，松材线虫的存活率显著提高，表明松材线虫耐寒性随着驯化时间的延长显著提高，但是0℃和5℃两种驯化温度对松材线虫的耐寒性增加差异不显著。
위료탐구송재선충적내한성，이채자호북성의창시이릉구적송재선충（ Bursaphelenchus xylophilus）위시험대상，연구저온급저온순화대송재선충존활정황적영향。결과표명：송재선충유교강적내저온능력，재10、5、0、－5、－10℃저온조건하，수착처리시간적연장，송재선충존활솔축점강저，각온도반치사시간분별위20．17、35．63、30．65、1．17 d，기중－10℃저온조건하단잠폭로，선충전부사망。차외，재0℃화5℃조건하경불동시간순화후，－5℃폭로처리24 h，송재선충적존활솔현저제고，표명송재선충내한성수착순화시간적연장현저제고，단시0℃화5℃량충순화온도대송재선충적내한성증가차이불현저。
In order to explore the cold tolerance of Bursaphelenchus xly ophilsu , we took B.xylpo hilus from Yiling District, Yichang City, Hubei Province to examine how low temperature and cold acclimation affected survival rate of B.xylophilus. B.xylophilus was relatively cold resistant with median level, and the survival rate decreased with the increase of cold expo-sure time.The median lethal time of B.xylophilus were 20.17, 35.63, 30.65 and 1.17 days at 10℃, 5℃, 0℃and -5℃, respectively, and all died at -10℃for short exposure.The survival rates of B.xylophilus were enhanced significantly at -5℃for 24 hours’ exposure after different time acclimation at 5℃and 0℃.The cold acclimation enhanced the cold tolerance of B.Xylophilus, and there was a positive correlation between the acclimation and cold tolerant, but there was no significant cold tolerant increase at 5℃and 0℃.