东北林业大学学报
東北林業大學學報
동북임업대학학보
JOURNAL OF NORTHEAST FORESTRY UNIVERSITY
2014年
11期
128-133
,共6页
刘晓露%古丽吉米拉·米吉提%王志英%吴红渠
劉曉露%古麗吉米拉·米吉提%王誌英%吳紅渠
류효로%고려길미랍·미길제%왕지영%오홍거
分月扇舟蛾%过氧化氢酶%超氧化物歧化酶%羧酸酯酶%谷胱甘肽-S-转移酶
分月扇舟蛾%過氧化氫酶%超氧化物歧化酶%羧痠酯酶%穀胱甘肽-S-轉移酶
분월선주아%과양화경매%초양화물기화매%최산지매%곡광감태-S-전이매
Clostera anastomosis%Catalase%Superoxide dismutase%Carboxylesterase%Glutathione-S-transferase
以分月扇舟蛾（ Clostera anasot mosis）3龄幼虫为研究对象，比较了几种杀虫剂对其离体保护酶（ SOD和CAT）和解毒酶（CarE和GST）活性的影响。结果表明：0．100 mg· L－1高效氯氟氰菊酯、0．010 mg· L－1吡虫啉、0．001 mg· L－1灭幼脲对CAT活性的抑制作用较强，抑制率分别为63．50％、68．89％和62．11％，而敌敌畏、氧化乐果对CAT活性的抑制作用较小。0．100 mg· L－1高效氯氟氰菊酯对SOD活性的抑制作用最强，抑制率为60．82％，氧化乐果对 SOD活性有较强的促进作用，激活率可达168．72％（10．000 mg· L－1）。10．000 mg· L－1敌敌畏对CarE的抑制作用最强，抑制率为52．63％，1．000 mg· L－1高效氯氟氰菊酯对CarE的激活作用最强，激活率为43．06％。供试杀虫剂对GST活性均有抑制作用，1．000 mg· L－1灭幼脲对GST抑制率可达51．75％。由此可见，离体保护酶和解毒酶的活性与杀虫剂的类型有直接关系，并且同一类型不同化学结构的杀虫剂对离体酶活性的影响存在显著差异。
以分月扇舟蛾（ Clostera anasot mosis）3齡幼蟲為研究對象，比較瞭幾種殺蟲劑對其離體保護酶（ SOD和CAT）和解毒酶（CarE和GST）活性的影響。結果錶明：0．100 mg· L－1高效氯氟氰菊酯、0．010 mg· L－1吡蟲啉、0．001 mg· L－1滅幼脲對CAT活性的抑製作用較彊，抑製率分彆為63．50％、68．89％和62．11％，而敵敵畏、氧化樂果對CAT活性的抑製作用較小。0．100 mg· L－1高效氯氟氰菊酯對SOD活性的抑製作用最彊，抑製率為60．82％，氧化樂果對 SOD活性有較彊的促進作用，激活率可達168．72％（10．000 mg· L－1）。10．000 mg· L－1敵敵畏對CarE的抑製作用最彊，抑製率為52．63％，1．000 mg· L－1高效氯氟氰菊酯對CarE的激活作用最彊，激活率為43．06％。供試殺蟲劑對GST活性均有抑製作用，1．000 mg· L－1滅幼脲對GST抑製率可達51．75％。由此可見，離體保護酶和解毒酶的活性與殺蟲劑的類型有直接關繫，併且同一類型不同化學結構的殺蟲劑對離體酶活性的影響存在顯著差異。
이분월선주아（ Clostera anasot mosis）3령유충위연구대상，비교료궤충살충제대기리체보호매（ SOD화CAT）화해독매（CarE화GST）활성적영향。결과표명：0．100 mg· L－1고효록불청국지、0．010 mg· L－1필충람、0．001 mg· L－1멸유뇨대CAT활성적억제작용교강，억제솔분별위63．50％、68．89％화62．11％，이활활외、양화악과대CAT활성적억제작용교소。0．100 mg· L－1고효록불청국지대SOD활성적억제작용최강，억제솔위60．82％，양화악과대 SOD활성유교강적촉진작용，격활솔가체168．72％（10．000 mg· L－1）。10．000 mg· L－1활활외대CarE적억제작용최강，억제솔위52．63％，1．000 mg· L－1고효록불청국지대CarE적격활작용최강，격활솔위43．06％。공시살충제대GST활성균유억제작용，1．000 mg· L－1멸유뇨대GST억제솔가체51．75％。유차가견，리체보호매화해독매적활성여살충제적류형유직접관계，병차동일류형불동화학결구적살충제대리체매활성적영향존재현저차이。
We studied the effects of several pesticides on the activities of SOD, CAT, CarE and GST in three instars of Clostera anastomosis.The activities of CAT were highly inhibited by 0.1 mg· L-1 lambda-cyhalothrin, 0.010 mg· L-1 imidacloprid, and 0.001 mg· L-1 diflubenzuron, and the inhabitation rate were 63.50%, 68.89%and 62.11%, respectively.However, the activities of CAT were lowest inhibited by dichlorvos and omethoate, and highest inhibited by 0.1 mg· L-1 lambda-cy-halothrin.The inhabitation rate of 0.100 mg· L-1 lambda-cyhalothrin was 60.82%.The activities of SOD were highly in-creased by 10.000 mg· L-1 omethoate, and the increasing rate was 168.72%.The activities of CarE were highly inhibited by 10.000 mg· L-1 dichlorvos, and the increasing rate was 52.63%.The activities of CarE was highest increased by 1.000 mg· L-1 lambda-cyhalothrin, and the increasing rate of 1.000 mg· L-1 lambda-cyhalothrin was 43.06%.The activities of GST were all inhibited by the tested pesticides and the inhabitation rate of 1.000 mg· L-1 lambda-cyhalothrin reached 51.75%. Thus, the activities of protective and detoxification enzymes have direct relation to the type of pesticides, and the effects on the activities of protective and detoxification enzymes are significant different by the same type of pesticides with different chemical structures.
