CAJ | 학술논문

以园林观赏竹种菲白竹为材料，采用人工模拟酸雨的方法研究不同强度酸雨（ pH＝4．0、pH＝3．0、pH＝2．0）胁迫对菲白竹生理特性的影响。结果表明：不同强度的酸雨胁迫均导致菲白竹叶片出现不同程度的伤害症状，受伤害程度随着酸雨pH值的降低而加剧。胁迫解除初期，叶片可溶性蛋白质量分数和叶绿素相对含量（SPAD值）均低于对照，而丙二醛（MDA）质量摩尔浓度，质膜透性以及超氧化物歧化酶（SOD）、过氧化物酶（POD）活性较对照出现不同程度的升高，变化幅度随胁迫强度的增加而增加；不同生理指标对胁迫的敏感程度有所不同，可溶性蛋白质量分数、叶绿素相对含量、质膜透性等可作为反映酸雨胁迫下菲白竹受伤害程度的重要依据。经过1个月左右的恢复期，pH＝4．0胁迫处理组的大多数指标能恢复至对照水平；pH＝3．0处理组有一定的恢复趋势，但恢复程度不明显；而pH＝2．0胁迫组的各项指标均不能恢复，且与对照差异显著（ P＜0．05）。说明菲白竹对模拟酸雨pH＝4．0的胁迫处理表现出较强的自我修复能力；对pH＝3．0酸雨胁迫具有一定的修复作用；但在pH＝2．0处理下，则产生不可逆伤害，无法通过自我调节恢复，这与外部形态的观察结果一致。高频度强酸雨（ pH＝2．0）处理下，菲白竹叶片伤害的主要原因可能是由过量H＋累积对植株造成的直接伤害引起的；而低酸度（ pH＝4．0）处理产生的生理伤害及其修复与活性氧代谢调节有关。
이완림관상죽충비백죽위재료，채용인공모의산우적방법연구불동강도산우（ pH＝4．0、pH＝3．0、pH＝2．0）협박대비백죽생리특성적영향。결과표명：불동강도적산우협박균도치비백죽협편출현불동정도적상해증상，수상해정도수착산우pH치적강저이가극。협박해제초기，협편가용성단백질량분수화협록소상대함량（SPAD치）균저우대조，이병이철（MDA）질량마이농도，질막투성이급초양화물기화매（SOD）、과양화물매（POD）활성교대조출현불동정도적승고，변화폭도수협박강도적증가이증가；불동생리지표대협박적민감정도유소불동，가용성단백질량분수、협록소상대함량、질막투성등가작위반영산우협박하비백죽수상해정도적중요의거。경과1개월좌우적회복기，pH＝4．0협박처리조적대다수지표능회복지대조수평；pH＝3．0처리조유일정적회복추세，단회복정도불명현；이pH＝2．0협박조적각항지표균불능회복，차여대조차이현저（ P＜0．05）。설명비백죽대모의산우pH＝4．0적협박처리표현출교강적자아수복능력；대pH＝3．0산우협박구유일정적수복작용；단재pH＝2．0처리하，칙산생불가역상해，무법통과자아조절회복，저여외부형태적관찰결과일치。고빈도강산우（ pH＝2．0）처리하，비백죽협편상해적주요원인가능시유과량H＋루적대식주조성적직접상해인기적；이저산도（ pH＝4．0）처리산생적생리상해급기수복여활성양대사조절유관。
Leaves of Pleioblastus fortunei, an ornamental bamboo species, were sprayed with simulated acid rain ( SAR) with different levels of pH ( 4.0, 3.0, 2.0) to study the effects of SAR on leaf physiological characteristics.The SAR with dif-ferent intensity could result in damage symptoms appeared on the leaves.The lower the pH value was, the severer the dam-age was.During the early stage of stress relief, the values of Pr and SPAD were below to control and MDA, MP, SOD and POD were higher than control, whose ranges of the change increased significantly as the stress became deeper.Pr, MDA and MP could play as an important basis reflecting the damage degrees under SAR stress because of the sensitivities to stress.After one month recovery, the most of indexes of pH of 4.0 could return to control.pH of 3.0 could recover but not obviously.All the indexes of pH of 2.0 could not return to control and with significant differences ( P<0.05) .P.fortunei can adapt to pH of 4.0 SAR stress and stand pH of 3.0 SAR in a certain degree, but suffers irreversible damage during pH of 2.0 SAR, which is agree with morphological observation.Under high frequent pH of 2.0 SAR, the leaf damage of P.for-tunei may be resulted directly from the accumulation of excessive H+.However, the physiological damage and repair pro-duced by pH of 4.0 SAR is related to the regulation of reactive oxygen species metabolism.