医药前沿
醫藥前沿
의약전연
YIAYAO QIANYAN
2014年
22期
15-16
,共2页
脑组织冷冻切片%固定液%组织收缩%染色质量
腦組織冷凍切片%固定液%組織收縮%染色質量
뇌조직냉동절편%고정액%조직수축%염색질량
Brain tissue frozen section%Fixative%Tissue shrinkage%Staining quality
目的:分析10%福尔马林、纯丙酮、95%乙醇、甲醇4种固定液对脑组织冷冻切片H-E染色质量的影响,筛选出一种脑组织冷冻切片的最佳固定液。方法收集手术室送检新鲜脑组织标本,经常规冷冻切片后放入不同固定液,进行H-E染色,观察脑组织冷冻切片细胞收缩程度和染色质量。结果甲醇组细胞收缩不明显,相对于其他组比较差异有统计学意义(P<0.05);甲醇组组织结构清晰,核浆染色鲜艳,对比度好,相对于其他组差异性显著(P<0.001)。结论甲醇固定脑组织染色效果好,使用方便,满足术中冷冻切片的快速、准确诊断,是脑组织冷冻切片的理想固定液。
目的:分析10%福爾馬林、純丙酮、95%乙醇、甲醇4種固定液對腦組織冷凍切片H-E染色質量的影響,篩選齣一種腦組織冷凍切片的最佳固定液。方法收集手術室送檢新鮮腦組織標本,經常規冷凍切片後放入不同固定液,進行H-E染色,觀察腦組織冷凍切片細胞收縮程度和染色質量。結果甲醇組細胞收縮不明顯,相對于其他組比較差異有統計學意義(P<0.05);甲醇組組織結構清晰,覈漿染色鮮豔,對比度好,相對于其他組差異性顯著(P<0.001)。結論甲醇固定腦組織染色效果好,使用方便,滿足術中冷凍切片的快速、準確診斷,是腦組織冷凍切片的理想固定液。
목적:분석10%복이마림、순병동、95%을순、갑순4충고정액대뇌조직냉동절편H-E염색질량적영향,사선출일충뇌조직냉동절편적최가고정액。방법수집수술실송검신선뇌조직표본,경상규냉동절편후방입불동고정액,진행H-E염색,관찰뇌조직냉동절편세포수축정도화염색질량。결과갑순조세포수축불명현,상대우기타조비교차이유통계학의의(P<0.05);갑순조조직결구청석,핵장염색선염,대비도호,상대우기타조차이성현저(P<0.001)。결론갑순고정뇌조직염색효과호,사용방편,만족술중냉동절편적쾌속、준학진단,시뇌조직냉동절편적이상고정액。
Objective:To screen the best fixative on brain tissue frozen section,we analysed that 4 fixatives including 10% formalin,pure acetone,95% ethanol,and methanol impact on the quality of H&E staining quality.Methods:Fresh brain tissue specimens were colected,and we made frozen sections in the different fixatives,and observed brain tissue shrinkage and dying quality with H&E staining. Results:Cel shrinkage of methanol group was significantly less than other groups (P<0.05); For methanol group,organization structure is more clear,the color is more bright and nuclear plasma contrast is higher,relative to other groups (P<0.001).Conclusion: methanol fixation method is effective on staining,easy to use,and contributing to the rapid and accurate diagnosis of intraoperative frozen section. Methanol is an ideal fixative for brain tissue frozen section.