中国当代医药
中國噹代醫藥
중국당대의약
PERSON
2014年
31期
12-14
,共3页
张于%李云%裴斐%周洪霞%徐胜%董伟%张立民%杨鹏%张宇新%司道文
張于%李雲%裴斐%週洪霞%徐勝%董偉%張立民%楊鵬%張宇新%司道文
장우%리운%배비%주홍하%서성%동위%장립민%양붕%장우신%사도문
黄体酮%脑损伤%半胱氨酸天冬氨酸蛋白酶-3
黃體酮%腦損傷%半胱氨痠天鼕氨痠蛋白酶-3
황체동%뇌손상%반광안산천동안산단백매-3
Progesterone%Traumatic brain injury%Cysteine aspartic acid protease-3
目的：观察脑损伤后神经细胞凋亡变化及黄体酮的保护作用。方法雄性SD大鼠随机分为假手术组、脑损伤组和黄体酮治疗组,大鼠脑损伤模型制作是利用改良的Feeney自由落体损伤装置。各组于伤后24、48、72 h取材。用免疫组织化学法观察大鼠皮质和海马CA1区半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)的表达水平变化。结果脑损伤组的Caspase-3阳性细胞数在皮质和海马CA1区较假手术组显著增加,黄体酮治疗组与脑损伤组比较,Caspase-3阳性细胞数明显减少,差异有统计学意义(P<0.05)。结论黄体酮抑制脑损伤后Caspase-3的表达可能是黄体酮脑保护作用机制之一。
目的：觀察腦損傷後神經細胞凋亡變化及黃體酮的保護作用。方法雄性SD大鼠隨機分為假手術組、腦損傷組和黃體酮治療組,大鼠腦損傷模型製作是利用改良的Feeney自由落體損傷裝置。各組于傷後24、48、72 h取材。用免疫組織化學法觀察大鼠皮質和海馬CA1區半胱氨痠天鼕氨痠蛋白酶-3(Caspase-3)的錶達水平變化。結果腦損傷組的Caspase-3暘性細胞數在皮質和海馬CA1區較假手術組顯著增加,黃體酮治療組與腦損傷組比較,Caspase-3暘性細胞數明顯減少,差異有統計學意義(P<0.05)。結論黃體酮抑製腦損傷後Caspase-3的錶達可能是黃體酮腦保護作用機製之一。
목적：관찰뇌손상후신경세포조망변화급황체동적보호작용。방법웅성SD대서수궤분위가수술조、뇌손상조화황체동치료조,대서뇌손상모형제작시이용개량적Feeney자유락체손상장치。각조우상후24、48、72 h취재。용면역조직화학법관찰대서피질화해마CA1구반광안산천동안산단백매-3(Caspase-3)적표체수평변화。결과뇌손상조적Caspase-3양성세포수재피질화해마CA1구교가수술조현저증가,황체동치료조여뇌손상조비교,Caspase-3양성세포수명현감소,차이유통계학의의(P<0.05)。결론황체동억제뇌손상후Caspase-3적표체가능시황체동뇌보호작용궤제지일。
Objective To investigate the change of nerve cell apoptosis after traumatic brain injury and protective role of progesterone. Methods Male Spraque-Dawley rats were randomly divided into the sham-operated group,the brain damage group and the progesterone (PROG) treatment group.Improved Feeney free fall injury apparatus was used to es-tablish the brain injury model in rats.The rats were killed respectively after 24,48,72 injury.The changes of the positive cell number of Caspase-3 in the cortex and hippocampus CA1 were examined by immunohistochemistry. Results The positive cell numbers of Caspase-3 of the brain damage group were increased. The positive cell numbers of Caspase-3 in the PROG treatment group were distinctly fewer than those of the brain damage group (P<0.05). Conclusion PROG inhibiting the expression of Caspase-3 after brain injury, may be one of the protective mechanisms of brain injury of PROG.
