CAJ | 학술논문

目的：探讨阿维A酸能否诱导原代人皮肤角质形成细胞凋亡及阿维A酸抗银屑病的作用机制。方法采用酶联免疫分析法、流式细胞术双标记法及透射电镜等方法观察药物作用细胞前后凋亡的诱导情况。结果阿维A酸可诱导原代人皮肤角质形成细胞凋亡，细胞凋亡程度呈现明显的浓度及时间依赖性。结论阿维A酸可诱导原代人皮肤角质形成细胞凋亡，阿维A酸治疗银屑病临床疗效肯定。
목적：탐토아유A산능부유도원대인피부각질형성세포조망급아유A산항은설병적작용궤제。방법채용매련면역분석법、류식세포술쌍표기법급투사전경등방법관찰약물작용세포전후조망적유도정황。결과아유A산가유도원대인피부각질형성세포조망，세포조망정도정현명현적농도급시간의뢰성。결론아유A산가유도원대인피부각질형성세포조망，아유A산치료은설병림상료효긍정。
Objective To investigate the acitretin A can be induced in primary human skin keratinocytes apoptosis and acitretin A anti psoriasis.Methods Flow cytometry double labeling and transmission electron microscopy were used to observe the apoptosis of the cells before and after induction of drug interaction analysis, by enzyme linked immunosorbent assay. Results Acitretin A can be induced in primary human skin keratinocytes apoptosis, cell apoptosis degree in a concentration and time obvious dependence.Conclusions The citretin A can induce the apoptosis of the primary human keratinocytes, and the citretin A has curative effect on the clinical curative effect of acitretin.