CAJ | 학술논문

目的：通过检测肝细胞肝癌（HCC）组织中microRNA-143（miR-143）的表达，探讨miR-143在HCC侵袭转移过程中的作用。方法收集45例HCC组织和对应的癌旁肝组织标本，采用实时荧光定量PCR（qPCR）法检测上述组织中miR-143的表达，并分析其与HCC的临床病理学参数及HBV的关系。结果癌旁肝组织中无miR-143表达的病例，HCC组织中miR-143的阳性表达率为88.89%（40/45）。miR-143的表达与肿瘤数目、肿瘤包膜、门静脉癌栓、癌细胞分化、远处转移和肿瘤分期呈显著相关（P均＜0.05），而与患者年龄、性别、肿瘤直径和HBV感染无关（P均＞0.05）；miR-143表达阳性与阴性的HCC患者3年生存率分别为89.12%、32.56%，差异有统计学意义（P ＜0.005）。结论 miR-143表达可能与HCC的侵袭转移有关，miR-143可作为预测HCC预后的标志物。
목적：통과검측간세포간암（HCC）조직중microRNA-143（miR-143）적표체，탐토miR-143재HCC침습전이과정중적작용。방법수집45례HCC조직화대응적암방간조직표본，채용실시형광정량PCR（qPCR）법검측상술조직중miR-143적표체，병분석기여HCC적림상병이학삼수급HBV적관계。결과암방간조직중무miR-143표체적병례，HCC조직중miR-143적양성표체솔위88.89%（40/45）。miR-143적표체여종류수목、종류포막、문정맥암전、암세포분화、원처전이화종류분기정현저상관（P균＜0.05），이여환자년령、성별、종류직경화HBV감염무관（P균＞0.05）；miR-143표체양성여음성적HCC환자3년생존솔분별위89.12%、32.56%，차이유통계학의의（P ＜0.005）。결론 miR-143표체가능여HCC적침습전이유관，miR-143가작위예측HCC예후적표지물。
Objective To explore the possible function and significance of miR-143 expression by detecting the miR-143 expression in carcinoma and the corresponding para-carcinoma tissue from patients with hepatocellular carcinoma. Methods Total of 45 samples of HCC and the corresponding para-carcinoma tissues were collected from our hospital, and the real-time quantitative reverse transcription (qRT-PCR) was used to test the miR-143 expression. The correlation between the miR-143 expression and the clinical pathological parameters of HCC was analyzed. Results There was no miR-143 expression in the corresponding para-carcinoma tissues, and the positive expressing rate of miR-143 in HCC tissues was 88.89% (40/45). It’s been showed positive correlation between the miR-143 expression and the tumor number, tumor capsule, portal vein tumor thrombus, tumor differentiation grade, distant metastasis and tumor stage (all P < 0.05), but no correlation between the miR-143 expression and the age, sexuality, tumor diameter, HBV infection (all P > 0.05). MiR-143 expression of positive and negative of HCC patients with 3 year survival rates were 89.12%, 32.56%, the difference was statistically significant (P < 0.005). Conclusions miR-143 may contribute to the invasion and metastasis of HCC and was an biological marker for predicting the prognosis of HCC.