CAJ | 학술논문

利用Micro－CT技术的较高分辨精度，探索一种新的测量小动物关节软骨形态的方法，为进一步利用小动物模型开展关节软骨的损伤和退变等研究提供基础。选择不同浓度的造影剂及Micro－CT扫描条件，通过造影剂对比增强Micro－CT扫描大鼠膝关节，对大鼠股骨远端关节软骨进行手动分割、重建，得到软骨3D模型，利用重建得到的3D模型，对软骨的厚度和体积等形态学参数进行无创定量分析。根据造影剂、软骨和软骨下骨对X射线吸收的平均相对衰减值差异，确定30％的离子造影剂浓度为最佳。利用该浓度对4个不同的正常大鼠关节软骨进行扫描和重建，分析获得关节软骨的体积和厚度，并求得关节软骨体积和厚度的变异系数均方根分别为7．3％和6．1％。结果提示，利用造影剂增强Micro－CT扫描能够对软骨形态重要参数厚度、体积等进行定量评价。
이용Micro－CT기술적교고분변정도，탐색일충신적측량소동물관절연골형태적방법，위진일보이용소동물모형개전관절연골적손상화퇴변등연구제공기출。선택불동농도적조영제급Micro－CT소묘조건，통과조영제대비증강Micro－CT소묘대서슬관절，대대서고골원단관절연골진행수동분할、중건，득도연골3D모형，이용중건득도적3D모형，대연골적후도화체적등형태학삼수진행무창정량분석。근거조영제、연골화연골하골대X사선흡수적평균상대쇠감치차이，학정30％적리자조영제농도위최가。이용해농도대4개불동적정상대서관절연골진행소묘화중건，분석획득관절연골적체적화후도，병구득관절연골체적화후도적변이계수균방근분별위7．3％화6．1％。결과제시，이용조영제증강Micro－CT소묘능구대연골형태중요삼수후도、체적등진행정량평개。
Based on the high resolution of Micro-CT, to explore a novel method for measuring the morphology of articular carti-lage in small animals, providing a basis to develop further research on the articular cartilage degeneration and injury using small ani-mals’ model.The different concentration of contrast agent and scanning condition of Micro-CT were selected.By an ionic contrast a-gent-enhanced micro-CT scanning of rat distal femur, the 3D model of articular cartilage was remodeled after segmentation and re-construction.And then, using this 3D model, the cartilage morphology in the rat such as thickness and volume was assessed quantita-tively and nondestructively.According to the different X-ray attenuation ( CT value) of contrast agent, cartilage and calcified bone, 30%contrast agent was determined as the optimal concentration.Under this condition, 4 rats′femurs were scanned and remodeled, the volume and thickness of articular cartilage were determined.The root-mean-square of coefficient variance for volume and thick-ness was 7.3%and 6.1%, respectively.Results show that using contrast agent enhanced Micro-CT scanning is an effective method to quantitatively evaluate the articular cartilage morphology including thickness and volume.