质量技术监督研究
質量技術鑑督研究
질량기술감독연구
QUALITY AND TECHNICAL SUPERVISION RESEARCH
2014年
5期
5-7,10
,共4页
油炸小食品%乙二胺四乙酸二钠%梯度洗脱%反相高效液相色谱法
油炸小食品%乙二胺四乙痠二鈉%梯度洗脫%反相高效液相色譜法
유작소식품%을이알사을산이납%제도세탈%반상고효액상색보법
Fried small foods%Disodium EDTA%Gradient elution%RP-HPLC
目的：文中建立一种测定油炸食品中乙二胺四乙酸二钠的液相色谱检测方法。方法油炸小食品经无水乙醚除油并挥去乙醚，水萃取，加入氯化铜溶液及抗坏血酸溶液形成Cu-EDTA络合物。色谱分离时使用C18分析柱，甲醇、四正丁基溴化铵缓冲液为流动相，梯度洗脱，245nm波长检测。结果该方法线性范围20.00μg?ml-1～400.00μg?ml-1，相关系数r：0.9999,检出限6.5mg?kg-1，定量限21mg?kg-1。回收率：93.7～100.7%；精密度： RSD60.2%。结论该方法的准确性和精密度满足检测要求需要。
目的：文中建立一種測定油炸食品中乙二胺四乙痠二鈉的液相色譜檢測方法。方法油炸小食品經無水乙醚除油併揮去乙醚，水萃取，加入氯化銅溶液及抗壞血痠溶液形成Cu-EDTA絡閤物。色譜分離時使用C18分析柱，甲醇、四正丁基溴化銨緩遲液為流動相，梯度洗脫，245nm波長檢測。結果該方法線性範圍20.00μg?ml-1～400.00μg?ml-1，相關繫數r：0.9999,檢齣限6.5mg?kg-1，定量限21mg?kg-1。迴收率：93.7～100.7%；精密度： RSD60.2%。結論該方法的準確性和精密度滿足檢測要求需要。
목적：문중건립일충측정유작식품중을이알사을산이납적액상색보검측방법。방법유작소식품경무수을미제유병휘거을미，수췌취，가입록화동용액급항배혈산용액형성Cu-EDTA락합물。색보분리시사용C18분석주，갑순、사정정기추화안완충액위류동상，제도세탈，245nm파장검측。결과해방법선성범위20.00μg?ml-1～400.00μg?ml-1，상관계수r：0.9999,검출한6.5mg?kg-1，정량한21mg?kg-1。회수솔：93.7～100.7%；정밀도： RSD60.2%。결론해방법적준학성화정밀도만족검측요구수요。
Objective:A method of determination of disodium EDTA of fried small foods by HPLC is established in this paper. Method:Through removing oil by anhydrous ether, evaporating ether subsequently, EDTA in fried small foods is then extracted by water. Addition of CuCl2 solution and ascorbic acid solution, then the Cu-EDTA complex combond can be formed. In the chromatographic separation, C18 analysis column is served as isolated column, methanol mixed with tetra-n-butylammonium bromide buffer solution(pH=4.0)is used as mobile phase, gradient elution program is made and the detection wavelength is 245 nm. Result: Linear range of the standard curve is 20.00μg ? ml-1~ 400.00μg ? ml-1with the correlation coefifcient r=0.9999. The recovery rate of the method is 93.7 ~ 100.7%.The detection limit is 6.5mg?kg-1 and the quantitative limit of the method is 21mg?kg-1. Conclusion Precision and accuracy of this method meets the requirements of the detection.
