中华损伤与修复杂志（电子版）
中華損傷與脩複雜誌（電子版）
중화손상여수복잡지（전자판）
Chinese Journal of Injury Repair and Wound Healing
2014年
5期
482-485
,共4页
冯国华%杨志宏%刘华%田诗政
馮國華%楊誌宏%劉華%田詩政
풍국화%양지굉%류화%전시정
骨髓祖代细胞%大鼠%外科皮瓣%血管生成
骨髓祖代細胞%大鼠%外科皮瓣%血管生成
골수조대세포%대서%외과피판%혈관생성
Myeloid progenitor cells%Rats%Surgical flaps%Angiogenesis
目的：观察骨髓基质干细胞（BMSCs）促进大鼠缺血皮瓣血管生成作用。方法从6只幼年雄性 SPF 级 SD 大鼠骨髓中分离获取 BMSCs，培养扩增后，取第3代 BMSCs。将48只成年 SPF 级SD 大鼠随机分成实验组和对照组，每组24只。在两组大鼠背部皮肤设计超长随意皮瓣，制作皮瓣模型，实验组大鼠注射重悬 BMSCs 于皮瓣远端，对照组大鼠同法注射等容积的完全培养液，两组大鼠分别于术后7、14 d 切取皮瓣远端标本行苏木精-伊红（HE）染色和免疫组织化学染色，检测皮瓣内微血管数及血管内皮生长因子（VEGF）的表达。结果实验组大鼠注射移植术后7、14 d VEGF 表达计分数（4.21±1.02、3.88±1.08）分别高于对照组术后7、14 d VEGF 表达计分数（2.58±0.97、2.29±1.08），两组比较差异均有统计学意义（t =5.642、5.082，P 均<0.05）。实验组术后7、14 d 微血管数（72.33±15.60、80.96±12.18）分别高于对照组术后7、14 d 微血管数（50.58±15.67、64.00±16.16），两组比较差异均有统计学意义（t =4.820、4.111，P 均<0.05）。结论 BMSCs 能分化内皮细胞成分，促进大鼠皮瓣血管新生。
目的：觀察骨髓基質榦細胞（BMSCs）促進大鼠缺血皮瓣血管生成作用。方法從6隻幼年雄性 SPF 級 SD 大鼠骨髓中分離穫取 BMSCs，培養擴增後，取第3代 BMSCs。將48隻成年 SPF 級SD 大鼠隨機分成實驗組和對照組，每組24隻。在兩組大鼠揹部皮膚設計超長隨意皮瓣，製作皮瓣模型，實驗組大鼠註射重懸 BMSCs 于皮瓣遠耑，對照組大鼠同法註射等容積的完全培養液，兩組大鼠分彆于術後7、14 d 切取皮瓣遠耑標本行囌木精-伊紅（HE）染色和免疫組織化學染色，檢測皮瓣內微血管數及血管內皮生長因子（VEGF）的錶達。結果實驗組大鼠註射移植術後7、14 d VEGF 錶達計分數（4.21±1.02、3.88±1.08）分彆高于對照組術後7、14 d VEGF 錶達計分數（2.58±0.97、2.29±1.08），兩組比較差異均有統計學意義（t =5.642、5.082，P 均<0.05）。實驗組術後7、14 d 微血管數（72.33±15.60、80.96±12.18）分彆高于對照組術後7、14 d 微血管數（50.58±15.67、64.00±16.16），兩組比較差異均有統計學意義（t =4.820、4.111，P 均<0.05）。結論 BMSCs 能分化內皮細胞成分，促進大鼠皮瓣血管新生。
목적：관찰골수기질간세포（BMSCs）촉진대서결혈피판혈관생성작용。방법종6지유년웅성 SPF 급 SD 대서골수중분리획취 BMSCs，배양확증후，취제3대 BMSCs。장48지성년 SPF 급SD 대서수궤분성실험조화대조조，매조24지。재량조대서배부피부설계초장수의피판，제작피판모형，실험조대서주사중현 BMSCs 우피판원단，대조조대서동법주사등용적적완전배양액，량조대서분별우술후7、14 d 절취피판원단표본행소목정-이홍（HE）염색화면역조직화학염색，검측피판내미혈관수급혈관내피생장인자（VEGF）적표체。결과실험조대서주사이식술후7、14 d VEGF 표체계분수（4.21±1.02、3.88±1.08）분별고우대조조술후7、14 d VEGF 표체계분수（2.58±0.97、2.29±1.08），량조비교차이균유통계학의의（t =5.642、5.082，P 균<0.05）。실험조술후7、14 d 미혈관수（72.33±15.60、80.96±12.18）분별고우대조조술후7、14 d 미혈관수（50.58±15.67、64.00±16.16），량조비교차이균유통계학의의（t =4.820、4.111，P 균<0.05）。결론 BMSCs 능분화내피세포성분，촉진대서피판혈관신생。
Objective To observe the effect of accelerating angiogenesis in ischemic flap with bone marrow-derived mesenchymal stem cells(BMSCs). Methods BMSCs were isolated from the marrow of Sprague-Dawley(SD)rats and cultured in vitro by adhering method,and the third passage cells were used in the experiment. The model of hyper-proportion flap was obtained on SD rat's back,and forty-eight SD rats were randomized equally into control group and experimental group. To contrast with the experimental group and the control group,which was treated by subcutaneously injected with BMSCs,the other group with injecting culture medium of BMSCs. The microvascular density and VEGF expression in flap specimens were measured with HE staining and immunohistochemistry in 7,14 postoperative days respectively. Results The VEGF expression levels of experimental group were 4. 21 ± 1. 02、3. 88 ± 1. 08 in 7,14 postoperative days respectively,and those in control group were 2. 58 ± 0. 97、2. 29 ± 1. 08. In experimental group,the microvascular density of flap specimens were 72. 33 ± 15. 60、80. 96 ± 12. 18 in 7,14 postoperative days respectively,and those in control group were 50. 58 ± 15. 67、64. 00 ± 16. 16. There were significant difference between experimental group and control group both in microvascular density and VEGF expression levels of flap specimens(P < 0. 05). Conclusions BMSCs can significantly enhance the angiogenesis of ischemic skin flap in rat.
