中国牛业科学
中國牛業科學
중국우업과학
CHINA CATTLE SCIENCE
2014年
5期
7-11
,共5页
刘霞%李积友%曾国敏%孙雪婧%韩登武%杜晓华
劉霞%李積友%曾國敏%孫雪婧%韓登武%杜曉華
류하%리적우%증국민%손설청%한등무%두효화
MSTN 基因%遗传多态性%西门塔尔牛
MSTN 基因%遺傳多態性%西門塔爾牛
MSTN 기인%유전다태성%서문탑이우
MSTN gene%genetic polymorphism%Simmental cattle
[目的]为分析金昌地区西门塔尔牛 MSTN 基因三个外显子的遗传多态性及变异特征。[方法]采用 PCR-SSCP 方法检测了61头西门塔尔牛 MSTN 基因三个外显子的多态性。[结果]显示：金昌西门塔尔牛 MSTN 基因的第1外显子存在 A、B 两个等位基因以及AA、AB 两种基因型，其基因型频率分别为0.7705和0.2295；第2外显子存在 A、B 两个等位基因以及 AA、AB、BB 三种基因型，其基因型频率分别为0.0328、0.3443和0.6229；第3外显子只有 AA 一种基因型。序列分析表明，金昌地区的西门塔尔牛 MSTN 基因第1外显子在269bp 发生了 A→G 的突变和第2外显子在41bp 发生了 C→T 的突变，但都未导致氨基酸发生改变，属于同义突变；外显子3并未检测到突变。[结论]统计结果表明，金昌地区的西门塔尔牛 MSTN 基因第1外显子呈低度多态，第2外显子呈中度多态。
[目的]為分析金昌地區西門塔爾牛 MSTN 基因三箇外顯子的遺傳多態性及變異特徵。[方法]採用 PCR-SSCP 方法檢測瞭61頭西門塔爾牛 MSTN 基因三箇外顯子的多態性。[結果]顯示：金昌西門塔爾牛 MSTN 基因的第1外顯子存在 A、B 兩箇等位基因以及AA、AB 兩種基因型，其基因型頻率分彆為0.7705和0.2295；第2外顯子存在 A、B 兩箇等位基因以及 AA、AB、BB 三種基因型，其基因型頻率分彆為0.0328、0.3443和0.6229；第3外顯子隻有 AA 一種基因型。序列分析錶明，金昌地區的西門塔爾牛 MSTN 基因第1外顯子在269bp 髮生瞭 A→G 的突變和第2外顯子在41bp 髮生瞭 C→T 的突變，但都未導緻氨基痠髮生改變，屬于同義突變；外顯子3併未檢測到突變。[結論]統計結果錶明，金昌地區的西門塔爾牛 MSTN 基因第1外顯子呈低度多態，第2外顯子呈中度多態。
[목적]위분석금창지구서문탑이우 MSTN 기인삼개외현자적유전다태성급변이특정。[방법]채용 PCR-SSCP 방법검측료61두서문탑이우 MSTN 기인삼개외현자적다태성。[결과]현시：금창서문탑이우 MSTN 기인적제1외현자존재 A、B 량개등위기인이급AA、AB 량충기인형，기기인형빈솔분별위0.7705화0.2295；제2외현자존재 A、B 량개등위기인이급 AA、AB、BB 삼충기인형，기기인형빈솔분별위0.0328、0.3443화0.6229；제3외현자지유 AA 일충기인형。서렬분석표명，금창지구적서문탑이우 MSTN 기인제1외현자재269bp 발생료 A→G 적돌변화제2외현자재41bp 발생료 C→T 적돌변，단도미도치안기산발생개변，속우동의돌변；외현자3병미검측도돌변。[결론]통계결과표명，금창지구적서문탑이우 MSTN 기인제1외현자정저도다태，제2외현자정중도다태。
Objective]In order to study the polymorphism in three exons within the MSTN gene in 61 Simmental cattle from Jinchang of Gansu province.[Method]DNA fragment containing the three exons within MSTN gene was amplified and genotyped using PCR single-strand conformation polymorphism (SS-CP)method.[Result]The results showed that the polymorphism in the exon 1 was controlled by A and B alleles that formed two genotypes,namely,AA and AB which possessed the frequencies at 0.7705 and 0. 2295,respectively.The polymorphism of exon 2 was controlled by A and B alleles,that formed three gen-otypes (AA,AB and BB)which possessed the frequencies at 0.0328,0.3443 and 0.6229,respectively. The polymorphism of exon 3 was controlled only by A allele which formed only one genotype (AA).Se-quence analysis indicated that there were two single nucleotide mutations including C to T at 41st nucleo-tide and A to G at 269th nucleotide in exon 2 and exon 1,respectively.Amino acid changes did not occur. However,there was no polymorphism in exon 3.[Conclusion]The overall results showed that the exon 1 had a low level of genetic diversity,while the exon 2 had moderate level of genetic diversity in Simmental cattle from Jinchang of Gansu province.
