CAJ | 학술논문

目的探讨脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)对高糖培养的大鼠海马神经元存活的保护作用.方法无血清体外培养的方法获得新生SD乳鼠海马神经元,分为正常组,高糖组及高糖BDNF组.用免疫荧光细胞化学的方法标记微管相关蛋白(Map2)检测培养的海马神经元的纯度和形态,用PI/Hoechest33342染色的方法检测神经元的存活率.结果大鼠海马神经元在高糖培养条件下存活率下降,易坏死或凋亡,存活率正常组:(96.11±1.63)％;高糖BDNF组:(93.47±2.43)％;高糖组:(76.29±6.74)％.高糖导致的大鼠海马神经元凋亡可以被BDNF抑制.结论 BDNF对高糖导致的大鼠海马神经元坏死或凋亡具有保护作用.
목적 탐토뇌원성신경영양인자(brain-derived neurotrophic factor,BDNF)대고당배양적대서해마신경원존활적보호작용.방법 무혈청체외배양적방법획득신생SD유서해마신경원,분위정상조,고당조급고당BDNF조.용면역형광세포화학적방법표기미관상관단백(Map2)검측배양적해마신경원적순도화형태,용PI/Hoechest33342염색적방법검측신경원적존활솔.결과 대서해마신경원재고당배양조건하존활솔하강,역배사혹조망,존활솔정상조:(96.11±1.63)％;고당BDNF조:(93.47±2.43)％;고당조:(76.29±6.74)％.고당도치적대서해마신경원조망가이피BDNF억제.결론 BDNF대고당도치적대서해마신경원배사혹조망구유보호작용.
Objective To investigate the effect of brain-derived neurotrophic factor on protection of rat hippocampal neurons after cultured with high glucose concentrations.Methods Hippocampal neurons of neonatal SD rat were cultured in serum-free medium and divided into normal group,high glucose group and high glucose BDNF group.The morphological changes of the neurons were observed under inverted fluorescence microscope.Immunofluorescence stain for Map2 was performed to identify the purity of neurons.The survival of the neuron were tested by PI/Hoechest33342 staining.Results Compared with the normal group [survival rate (96.11 ± 1.63) ％],the number of PI positive neurons were significantly increased in the high glucose group [survival rate (76.29 ± 6.74) ％].Treatment with BDNF [survival rate (93.47 ±2.43) ％] significantly reduced the number of PI positive cells.Conclusion BDNF can protect rat hippocampal neurons from apoptosis induced by high glucose.