南京林业大学学报(自然科学版)
南京林業大學學報(自然科學版)
남경임업대학학보(자연과학판)
JOURNAL OF NANJING FORESTRY UNIVERSITY(NATURAL SCIENCE EDITION)
2014年
6期
55-59
,共5页
李玲%马玉堃%王轶%刘雪峰
李玲%馬玉堃%王軼%劉雪峰
리령%마옥곤%왕질%류설봉
伴生真菌%青杨脊虎天牛%致病性%杨树
伴生真菌%青楊脊虎天牛%緻病性%楊樹
반생진균%청양척호천우%치병성%양수
associated fungi%Xylotrechus rusticus L.%pathogenicity%poplar
为了探索青杨脊虎天牛(Xylotrechus rusticus L.)伴生真菌群落组成及其对杨树的致病性,利用稀释平板涂布法进行伴生真菌的分离、培养,从青杨脊虎天牛(X. rusticus L.)各虫态、寄主及其蛀屑等9种材料中共分离出72个菌株,分属于19个菌属。青杨脊虎天牛蛀入寄主杨树后,可以引起寄主的树皮以及木质部坑道内真菌群落发生明显变化。与健康杨树相比,青杨脊虎天牛虫体及其蛀道内真菌组成、优势菌种类型都存在明显差异。研究结果证明,茄病镰刀菌(Fusarium solani (Mart.) Sacc.)是青杨脊虎天牛幼虫、蛹及其蛀害树皮表面存在的优势菌种,该优势菌培养液对杨树水培枝条具有致病性,且随着作用时间延长,致病性增强。
為瞭探索青楊脊虎天牛(Xylotrechus rusticus L.)伴生真菌群落組成及其對楊樹的緻病性,利用稀釋平闆塗佈法進行伴生真菌的分離、培養,從青楊脊虎天牛(X. rusticus L.)各蟲態、寄主及其蛀屑等9種材料中共分離齣72箇菌株,分屬于19箇菌屬。青楊脊虎天牛蛀入寄主楊樹後,可以引起寄主的樹皮以及木質部坑道內真菌群落髮生明顯變化。與健康楊樹相比,青楊脊虎天牛蟲體及其蛀道內真菌組成、優勢菌種類型都存在明顯差異。研究結果證明,茄病鐮刀菌(Fusarium solani (Mart.) Sacc.)是青楊脊虎天牛幼蟲、蛹及其蛀害樹皮錶麵存在的優勢菌種,該優勢菌培養液對楊樹水培枝條具有緻病性,且隨著作用時間延長,緻病性增彊。
위료탐색청양척호천우(Xylotrechus rusticus L.)반생진균군락조성급기대양수적치병성,이용희석평판도포법진행반생진균적분리、배양,종청양척호천우(X. rusticus L.)각충태、기주급기주설등9충재료중공분리출72개균주,분속우19개균속。청양척호천우주입기주양수후,가이인기기주적수피이급목질부갱도내진균군락발생명현변화。여건강양수상비,청양척호천우충체급기주도내진균조성、우세균충류형도존재명현차이。연구결과증명,가병렴도균(Fusarium solani (Mart.) Sacc.)시청양척호천우유충、용급기주해수피표면존재적우세균충,해우세균배양액대양수수배지조구유치병성,차수착작용시간연장,치병성증강。
In order to explore Xylotrchus rusticus L. associated fugal community composition and pathogenicity, dilution plate method was used to isolate fungi from X. rusticus L. instar, host frass that nine kinds of materials, and there were 72 strains which belonged to 19 genera. After X. rusticus bore into the host poplar, fungi could cause significant changes within the host community bark and xylem sap. Compared with healthy poplar, X. rusticus and fungi within the bore path composition and dominant strains types were significantly different. Our study showed that Fusarrium solani (Mart.) Sacc was Xylotechus beetle larvae, paupae and termite damage its dominant bacteria presented on the surface of bark. The dominant bacteria culture medium for hydroponic poplar branches had pathogenic, and with prolonged duration of action which enhanced pathogencity.