CAJ | 학술논문

选取来自中国、日本和美国的松材线虫（ Bursaphelenchus xylophilus）4个虫株，以及中国的拟松材线虫（ B． mucronatus）2个虫株进行生物学杂交和交配对象选择试验。结果表明：松材线虫和拟松材线虫的部分虫株可以发生种间杂交，正交总交配成功率为4?2％，反交总交配成功率为10％，反交成功率是正交的2倍。杂交整体发生率极低，杂交后代较难形成可持续的繁殖种群。采用松材线虫（简称Bx）/拟松材线虫（简称Bm）快速分子检测系统对杂交F1代幼虫进行分子鉴定，结果表明F1代幼虫既含有Bx分子标记也含有Bm分子标记。继代培养30 d后，杂交后代在基因型上表现出了不同趋向的分化，其中BxAN5♀×Bm JNL1♂、BxAN5♂×BmAN5♀和Bx?USA♂×Bm JNL1♀的杂交后代趋向于成为松材线虫占主导的种群，BxJNL5♂×BmJNL1♀的杂交后代趋向于成为拟松材线虫占主导的种群。在有种内交配资源存在情况下，无论松材线虫还是拟松材线虫均倾向于优先选择种内交配资源。研究表明松材线虫和拟松材线虫之间存在一定的生殖隔离，自然状态下种间交配可能会产生，但杂交后代较难形成可持续性繁殖的生殖后代。
선취래자중국、일본화미국적송재선충（ Bursaphelenchus xylophilus）4개충주，이급중국적의송재선충（ B． mucronatus）2개충주진행생물학잡교화교배대상선택시험。결과표명：송재선충화의송재선충적부분충주가이발생충간잡교，정교총교배성공솔위4?2％，반교총교배성공솔위10％，반교성공솔시정교적2배。잡교정체발생솔겁저，잡교후대교난형성가지속적번식충군。채용송재선충（간칭Bx）/의송재선충（간칭Bm）쾌속분자검측계통대잡교F1대유충진행분자감정，결과표명F1대유충기함유Bx분자표기야함유Bm분자표기。계대배양30 d후，잡교후대재기인형상표현출료불동추향적분화，기중BxAN5♀×Bm JNL1♂、BxAN5♂×BmAN5♀화Bx?USA♂×Bm JNL1♀적잡교후대추향우성위송재선충점주도적충군，BxJNL5♂×BmJNL1♀적잡교후대추향우성위의송재선충점주도적충군。재유충내교배자원존재정황하，무론송재선충환시의송재선충균경향우우선선택충내교배자원。연구표명송재선충화의송재선충지간존재일정적생식격리，자연상태하충간교배가능회산생，단잡교후대교난형성가지속성번식적생식후대。
In this study, the hybridization was conducted between four Bursaphelenchus xylophilus strains selected from China, Japan, the United States and two B. mucronatus strains selected from China. The results showed that, hybridiza?tion could occur between some B. xylophilus strains and B. mucronatus strains, the success rate of negative crossing was two times that of positive crossing,but hybridization rate was extremely low, and the hybrid offspring was difficult to form a sustainable breeding population. The F1 hybrids larvae contained both Bx molecular markers and Bm molecular markers by using Bx/Bm quick molecular detection system. Inter?specific hybrids showed different gene types after subculturing 30 days, the offspring nematodes of BxAN5♀×Bm JNL1♂,BxAN5♂×BmAN5♀ and BxUSA♂×Bm JNL1♀ tended to become the populations which dominant species was Bx, as well as the offspring of BxJNL5♂×BmJNL1♀tended to be?come the population which dominant species was Bm. If intraspecific matings could happen, both B. mucronatus and B. xylophilus were inclined to have intraspecific mating priority. The adaptability of B. xylophilus in mating was better. There was little possibility of reproductive isolation between Bx and Bm which geographically close, and their inter?specific hy?brids were easily to produce. The reproductive isolation existed between B. mucronatus and B. xylophilus, and introgres?sion might happen but hybrids were difficult to form a sustainable reproductive offspring.