CAJ | 학술논문

诱导多潜能干细胞拥有胚胎干细胞所有特征,包括多能性和生成各种体细胞.用皮肤细胞产生诱导多潜能干细胞,不仅起始细胞易获取,而且这些诱导多潜能干细胞更容易定向分化为角质形成细胞、黑素细胞和成纤维细胞等多种功能性皮肤细胞.患者自体来源的诱导多潜能干细胞是细胞疗法理想的细胞库,用诱导多潜能干细胞分化后的细胞治疗皮肤病,不仅细胞量充足,且可避免伦理问题和免疫排斥反应.利用回复突变体嵌合体,结合诱导多潜能干细胞技术,能获得充分的患者特异性功能性回复体细胞而用于治疗遗传性皮肤病.该技术可避免常规基因治疗中出现的免疫排斥和插入诱变.
유도다잠능간세포옹유배태간세포소유특정,포괄다능성화생성각충체세포.용피부세포산생유도다잠능간세포,불부기시세포역획취,이차저사유도다잠능간세포경용역정향분화위각질형성세포、흑소세포화성섬유세포등다충공능성피부세포.환자자체래원적유도다잠능간세포시세포요법이상적세포고,용유도다잠능간세포분화후적세포치료피부병,불부세포량충족,차가피면윤리문제화면역배척반응.이용회복돌변체감합체,결합유도다잠능간세포기술,능획득충분적환자특이성공능성회복체세포이용우치료유전성피부병.해기술가피면상규기인치료중출현적면역배척화삽입유변.
Induced pluripotent stem cells (iPSCs) have all the characteristics of embryonic stem cells (ESCs),including pluripotency and the potential to differentiate into various somatic cells.The iPSCs originating from skin not only can be easily obtained,but also are more liable to directly differentiate into functional skin cells such as keratinocytes,melanocytes and fibroblasts.Autologous iPSCs have been considered as an ideal cell bank for cell therapy,because they have a sufficient source,and allow the avoidance of related ethical issues and immunologic rejection.Revertant mosaicism can be used in combination with iPSC technology to obtain sufficient functional patient-specific revertant cells for genodermatosis,which allows the avoidance of immunologic rejection and insertional mutagenesis observed in traditional gene therapy.