生态环境学报
生態環境學報
생태배경학보
ECOLOGY AND ENVIRONMENT
2014年
10期
1650-1656
,共7页
MC%栗酒裂殖酵母%肝细胞%神经细胞%细胞毒性%基因损伤
MC%慄酒裂殖酵母%肝細胞%神經細胞%細胞毒性%基因損傷
MC%률주렬식효모%간세포%신경세포%세포독성%기인손상
microcystin%S.pombe%HepG2%U87%cytotoxicity%gene damage
主要探讨微囊藻毒素对真菌和哺乳动物细胞的毒害效果，以及其作为水体生理毒害评价模式细胞的可能。通过毒性试验和单细胞凝胶电泳实验，利用栗酒裂殖酵母细胞（S.pombe），人肝癌细胞（HepG2）和人脑星形胶质母细胞癌细胞（U87）3种生物模型评估MC对细胞DNA的损伤以及对细胞内氧化自由基水平的影响。并利用栗酒裂殖酵母的基因缺失体，判断MC所诱导的酵母细胞氧化应激路径。研究表明MC通过氧化路径诱导DNA损伤；缺失wis1△和pap1△基因的S.pombe细胞无法诱导ROS生产，wis1△和pap1△缺失型S.pombe细胞在40 mg·L-1-1MC暴露24 h下细胞增殖率仅下降7.3%、7.7%；氧化损伤是MC损伤S.pombe的主要方式；HepG2细胞和U87细胞较S.pombe细胞适于研究、评估MC的毒性。
主要探討微囊藻毒素對真菌和哺乳動物細胞的毒害效果，以及其作為水體生理毒害評價模式細胞的可能。通過毒性試驗和單細胞凝膠電泳實驗，利用慄酒裂殖酵母細胞（S.pombe），人肝癌細胞（HepG2）和人腦星形膠質母細胞癌細胞（U87）3種生物模型評估MC對細胞DNA的損傷以及對細胞內氧化自由基水平的影響。併利用慄酒裂殖酵母的基因缺失體，判斷MC所誘導的酵母細胞氧化應激路徑。研究錶明MC通過氧化路徑誘導DNA損傷；缺失wis1△和pap1△基因的S.pombe細胞無法誘導ROS生產，wis1△和pap1△缺失型S.pombe細胞在40 mg·L-1-1MC暴露24 h下細胞增殖率僅下降7.3%、7.7%；氧化損傷是MC損傷S.pombe的主要方式；HepG2細胞和U87細胞較S.pombe細胞適于研究、評估MC的毒性。
주요탐토미낭조독소대진균화포유동물세포적독해효과，이급기작위수체생리독해평개모식세포적가능。통과독성시험화단세포응효전영실험，이용률주렬식효모세포（S.pombe），인간암세포（HepG2）화인뇌성형효질모세포암세포（U87）3충생물모형평고MC대세포DNA적손상이급대세포내양화자유기수평적영향。병이용률주렬식효모적기인결실체，판단MC소유도적효모세포양화응격로경。연구표명MC통과양화로경유도DNA손상；결실wis1△화pap1△기인적S.pombe세포무법유도ROS생산，wis1△화pap1△결실형S.pombe세포재40 mg·L-1-1MC폭로24 h하세포증식솔부하강7.3%、7.7%；양화손상시MC손상S.pombe적주요방식；HepG2세포화U87세포교S.pombe세포괄우연구、평고MC적독성。
s:To research the toxic effect of microcystin exerting to fungus and mammalian cells, and to explore the potential model cell for biological toxicity assessment of eutrophication water body, several biological indicators are defined in this study to test the influence of MC on DNA damage and oxyradical concentrations includingS.pombe cells, HepG2 cells and U87 cells by toxicity tests and single cell gel electrophoresis. Moreover, application of genetically depletedS.pombe is efficient for determining the oxidative stress path induced by MC in/between yeast cells. Through the observation, we can see DNA damage by MC-LR was supposed to happen in oxidative path. The experiment shows thatS.pombe cells without genewis1△ andpap1△ can not induce ROS. Treated with 40 mg·L-1MC for 24 h, the growth rate of genewis1△ andpap1△ deletion mutant merely decline by 7.3%, 7.7%. Oxidative injury is the main MC damage exerting toS.pome cells. The HepG2 cells and U87 cells were more suitable for risk assessment than S.pombe cells.
