CAJ | 학술논문

为建立高效液相色谱法测定无核白葡萄果实中赤霉素含量(残留)的方法，样品采用冷甲醇萃取，超声波提取，离心后上清液经0.45 um 滤膜过滤，滤液用 HPLC 紫外检测器测定。色谱条件为以 A(0.05%磷酸水溶液)/B(乙腈)(A / B：80%/20%)为流动相，ZORBAX Extend C18柱，柱温为30℃，紫外检测器波长210 nm。结果表明：该法线性良好，相关系数r＞0.999，精密度在0.86%～3.43%范围之内，平均回收率89.83%。本方法快速、可靠、灵敏，可为测定无核白葡萄果实中赤霉素含量提供参考。
위건립고효액상색보법측정무핵백포도과실중적매소함량(잔류)적방법，양품채용랭갑순췌취，초성파제취，리심후상청액경0.45 um 려막과려，려액용 HPLC 자외검측기측정。색보조건위이 A(0.05%린산수용액)/B(을정)(A / B：80%/20%)위류동상，ZORBAX Extend C18주，주온위30℃，자외검측기파장210 nm。결과표명：해법선성량호，상관계수r＞0.999，정밀도재0.86%～3.43%범위지내，평균회수솔89.83%。본방법쾌속、가고、령민，가위측정무핵백포도과실중적매소함량제공삼고。
Objective] to establish HPLC determination of gibberellin content in seedless grape fruit chromatography (residual) method. [Methods] sample using cold methanol extraction, ultrasonic extraction, centrifugation supernatant by 0.45um membrane filtration, filtrate was determined by HPLC UV detector. The chromatographic conditions were A (0.05% aqueous solution of phosphoric acid)/B (Yi Jing) (A/B:80%/20%) as the mobile phase, the ZORBAX Extend C18 column, the column temperature was 30 ℃ , UV detection wavelength was 210 nm. [results] the method with good linearity, correlation coefficient r>0.999, precision in the range of 0.86%-3.43%, the average recovery rate was 89.83%. [Conclusion] the method is rapid, reliable, sensitive, which can provide reference for the determination of gibberellin content in seedless grape fruit.