牙体牙髓牙周病学杂志
牙體牙髓牙週病學雜誌
아체아수아주병학잡지
CHINESE JOURNAL OF CONSERVATIVE DENTISTRY
2014年
11期
642-644
,共3页
卢婧%徐星星%李鑫%贾珍%唐荣银%王菁
盧婧%徐星星%李鑫%賈珍%唐榮銀%王菁
로청%서성성%리흠%가진%당영은%왕정
五倍子水提取物%体外培养%人牙髓细胞%Ⅰ型胶原
五倍子水提取物%體外培養%人牙髓細胞%Ⅰ型膠原
오배자수제취물%체외배양%인아수세포%Ⅰ형효원
gallnut water extracts%in vitro culture%human dental pulp cells%collagen I
目的:观察五倍子水提取物对人牙髓细胞Ⅰ型胶原合成的影响。方法:取第6代体外培养的人牙髓细胞分别与终末浓度为5、10μg/mL的五倍子水提取物共同培养3 d后,采用免疫组化染色法观察人牙髓细胞中Ⅰ型胶原的表达情况,并用HPIAS-1000图像分析系统进行定量分析。结果:对照组和不同浓度五倍子水提取物作用的实验组人牙髓细胞中均有Ⅰ型胶原的表达,其免疫反应物质多定位于胞浆。人牙髓细胞经5、10μg/mL浓度的五倍子水提取物作用后,其Ⅰ型胶原的表达水平均明显增高,其中以10μg/mL组的增高程度最大,各组间两两相比差异均有统计学意义(P<0.05)。结论:5、10μg/mL五倍子水提取物均能明显促进人牙髓细胞中Ⅰ型胶原的表达;提示其具有促进牙髓细胞向成牙本质细胞分化的作用。
目的:觀察五倍子水提取物對人牙髓細胞Ⅰ型膠原閤成的影響。方法:取第6代體外培養的人牙髓細胞分彆與終末濃度為5、10μg/mL的五倍子水提取物共同培養3 d後,採用免疫組化染色法觀察人牙髓細胞中Ⅰ型膠原的錶達情況,併用HPIAS-1000圖像分析繫統進行定量分析。結果:對照組和不同濃度五倍子水提取物作用的實驗組人牙髓細胞中均有Ⅰ型膠原的錶達,其免疫反應物質多定位于胞漿。人牙髓細胞經5、10μg/mL濃度的五倍子水提取物作用後,其Ⅰ型膠原的錶達水平均明顯增高,其中以10μg/mL組的增高程度最大,各組間兩兩相比差異均有統計學意義(P<0.05)。結論:5、10μg/mL五倍子水提取物均能明顯促進人牙髓細胞中Ⅰ型膠原的錶達;提示其具有促進牙髓細胞嚮成牙本質細胞分化的作用。
목적:관찰오배자수제취물대인아수세포Ⅰ형효원합성적영향。방법:취제6대체외배양적인아수세포분별여종말농도위5、10μg/mL적오배자수제취물공동배양3 d후,채용면역조화염색법관찰인아수세포중Ⅰ형효원적표체정황,병용HPIAS-1000도상분석계통진행정량분석。결과:대조조화불동농도오배자수제취물작용적실험조인아수세포중균유Ⅰ형효원적표체,기면역반응물질다정위우포장。인아수세포경5、10μg/mL농도적오배자수제취물작용후,기Ⅰ형효원적표체수평균명현증고,기중이10μg/mL조적증고정도최대,각조간량량상비차이균유통계학의의(P<0.05)。결론:5、10μg/mL오배자수제취물균능명현촉진인아수세포중Ⅰ형효원적표체;제시기구유촉진아수세포향성아본질세포분화적작용。
AIM:To observe the effects of gallnut water extracts on collagen I synthesis of human dental pulp cells.METHODS:Cultured human dental pulp cells of passage 6 were treated with 5 μg/mL and 10 μg/mL of gallnut water extracts for 3 d respectively.Collagen I expression of the cells was detected by immunocytochemistry method.The quantitative data was obtained by HPIAS-1000 image analysis system.RESULTS:Collagen I protein was expressed in of both gallnut water extract treated dental pulp cells and control cells,and located mainly in cyto-plasm.Gallnut water extracts treatment dose-dependently increased collagen I protein expression (P<0.05).CON-CLUSION:Both 5 μg/mL and 10 μg/mL gallnut water extracts can enhance collagen I protein expression in human dental pulp cells,indicating that it is potential to promote dental pulp cells to differentiate into odontoblasts.