CAJ | 학술논문

目的：通过静电纺丝技术制备载不同含量布洛芬（IPF）的聚乳酸－羟基乙酸共聚物（PL-GA）/聚乳酸（PLA）纳米纤维引导组织再生（GTR）膜，评价其生物学性能。方法：使用静电纺丝技术制备PLA纳米纤维膜，扫描电镜观察其表面形貌，与EMT－6细胞复合培养，检测其生物相容性及机械阻挡功能；制备载不同含量布洛芬的PLGA/PLA纳米纤维膜，观察其表面形貌、MTT法检测人牙龈成纤维细胞（HGFs）在其上的增殖情况，同时检测其载药率及药物缓释情况。结果：PLA以及载入不同量IPF的电纺纤维的直径均为300 nm～5μm，载入布洛芬后，纳米纤维仍表面光滑、均一、连续；绝大多数的EMT－6细胞均黏附在PLA膜的表面，膜内部几乎没有细胞；HGFs与不同载药量的IPF－PLG/PLA电纺膜复合培养1、3、5、7 d时，各载药实验组在各时间点的OD值与空白对照组相比 P＞0．05；5％、10％、15％3种载药量的电纺膜的载药率均可达85％以上，在1周内的突释量分别为30％、60％、85％，突释较小，药物释放时间可持续10～28 d。结论：本实验所制备的载布洛芬PLGA/PLA电纺纳米纤维膜具有良好生物相容性、优异的阻挡功能及缓慢释药功能。
목적：통과정전방사기술제비재불동함량포락분（IPF）적취유산－간기을산공취물（PL-GA）/취유산（PLA）납미섬유인도조직재생（GTR）막，평개기생물학성능。방법：사용정전방사기술제비PLA납미섬유막，소묘전경관찰기표면형모，여EMT－6세포복합배양，검측기생물상용성급궤계조당공능；제비재불동함량포락분적PLGA/PLA납미섬유막，관찰기표면형모、MTT법검측인아간성섬유세포（HGFs）재기상적증식정황，동시검측기재약솔급약물완석정황。결과：PLA이급재입불동량IPF적전방섬유적직경균위300 nm～5μm，재입포락분후，납미섬유잉표면광활、균일、련속；절대다수적EMT－6세포균점부재PLA막적표면，막내부궤호몰유세포；HGFs여불동재약량적IPF－PLG/PLA전방막복합배양1、3、5、7 d시，각재약실험조재각시간점적OD치여공백대조조상비 P＞0．05；5％、10％、15％3충재약량적전방막적재약솔균가체85％이상，재1주내적돌석량분별위30％、60％、85％，돌석교소，약물석방시간가지속10～28 d。결론：본실험소제비적재포락분PLGA/PLA전방납미섬유막구유량호생물상용성、우이적조당공능급완만석약공능。
AIM:To investigate the biological performance of ibuprofen(IPF)-loaded electrospun poly lac-tic-co-glycolic acid (PLGA)/poly lactic acid (PLA)nanofibrous guided tissue regeneration membrane.METH-ODS:PLA nanofibrous membranes and IPF(at 5%,10% and 15% respectively)-loaded PLGA/PLA (IPF-PL-GA/PLA)nanofibrous membranes were synthesized using an electrospinning technique.The morphology of the nanofi-bers was observed under SEM.Human gingival fibroblasts were seeded on IPF -PLGA/PLA membranes and HGFs proliferation was assessed with MTT assay.Drug entrapment efficiency was determined and drug release profile was tested.RESULTS:The diameters of the nanofibers were 300 nm -5 μm,and the IPF -loaded nanofibers were smooth,uniform and continuous.The majority of EMT-6 cells adhered on the surface of PLA membranes,while almost no cells appeared in the membranes.MTT assay showed that the number of EMT-6 cells of the experimental group was not significantly different from that of the control group after 1,3,5 and 7 day culture(P>0.05).Ibuprofen en-trapment efficiencies were above 85%for all the IPF-loaded membranes.The release of ibuprofen at 5%、10%、15%wrapped in fiber was 30%,60%and 85%respectively in 7 days and the release was going on for 10 -28 days.CON-CLUSION:IPF-PLGA/PLA nanofibrous membranes are biocompatible with excellent barrier function for cells and slow drug release.