CAJ | 학술논문

目的：探讨牙本质屏障法用于牙齿充填材料体外细胞毒性试验测定的特点。方法：低速锯片切新鲜拔除的磨牙，获取紧邻牙髓的颊侧牙本质片，选择通透值相近的牙本质片作为牙本质屏障，制作细胞毒性体外测试装置，用此装置测定氧化锌丁香油水门汀、复合树脂、玻璃离子水门汀、氢氧化钙水门汀、磷酸锌水门汀及阴性对照材料和阳性对照材料的细胞毒性，同时用传统的浸提液法测定这5种材料的细胞毒性。结果：牙本质屏障法测定的5种材料的细胞相对活性（％）分别为89．1±3．6、81．8±3．7、96．4±2．8、96．2±1．8、74．5±2．8；浸提液法测定的5种材料的浸提原液细胞相对活性（％）分别为35．6±5．2、72．9±4．3、91．5±1．3、90．2±2．6、59．3±2．6。结论：牙本质屏障可以显著减少充填材料对细胞的毒性作用，牙本质屏障法更能反映充填材料的体内细胞毒性。
목적：탐토아본질병장법용우아치충전재료체외세포독성시험측정적특점。방법：저속거편절신선발제적마아，획취긴린아수적협측아본질편，선택통투치상근적아본질편작위아본질병장，제작세포독성체외측시장치，용차장치측정양화자정향유수문정、복합수지、파리리자수문정、경양화개수문정、린산자수문정급음성대조재료화양성대조재료적세포독성，동시용전통적침제액법측정저5충재료적세포독성。결과：아본질병장법측정적5충재료적세포상대활성（％）분별위89．1±3．6、81．8±3．7、96．4±2．8、96．2±1．8、74．5±2．8；침제액법측정적5충재료적침제원액세포상대활성（％）분별위35．6±5．2、72．9±4．3、91．5±1．3、90．2±2．6、59．3±2．6。결론：아본질병장가이현저감소충전재료대세포적독성작용，아본질병장법경능반영충전재료적체내세포독성。
AIM:To compare cell reaction to five common dental filling materials tested with an indirect contact method equipped with dentin barrier and a conventional extraction culture method.METHODS:Dentin discs with 0.5mm in thickness were cut from the buccal inner dentin of freshly extracted molars and were determined for hy-draulic permeability.The discs with similar permeability were used as barrier to prepare device for cytotoxicity test in vitro.Cytotoxicity of composite resin,ZOE,GIC,Ca(OH)2 and ZPC cement,was tested using both this device and conventional extraction culture method with MTT assay.RESULTS:Relative cell viability (RCV,%)obtained with dentin barrier for the five filling materials were:81.8 ±3.7,89.1 ±3.6,96.4 ±2.8,96.2 ±1.8 and 74.5 ±2.8 re-spectively.The RCV(%)obtained with extraction culture method were 72.9 ±4.3,35.6 ±5.2,91.5 ±1.3,90.2 ± 2.6 and 59.3 ±2.6 respectively.CONCLUSION:Dentin barrier reduced the toxicity of filling material to cells,and the results from dentin barrier method are more reliable in reflecting the in vivo toxicity of filling materials.