热带农业科学
熱帶農業科學
열대농업과학
CHINESE JOURNAL OF TROPICAL AGRICULTURE
2014年
11期
71-74
,共4页
热带亚热带水果%胶孢炭疽菌%多菌灵%抗药性
熱帶亞熱帶水果%膠孢炭疽菌%多菌靈%抗藥性
열대아열대수과%효포탄저균%다균령%항약성
tropical and subtropical fruits%Colletotrichum gloeosporioides%carbendazim%drug resistance
采用生长速率法对来自海南18种热带亚热带水果的893株胶孢炭疽菌(C. gloeosporioides)进行多菌灵抗药性测定。结果表明:在供试菌株中,11.87%的菌株对多菌灵产生了不同程度的抗性,且高抗菌株达9.63%,具有抗多菌灵的 C. gloeosporioides 菌株主要来自芒果、荔枝和龙眼等14种水果。多菌灵对供试菌株的毒力存在差异, EC50值的最高值与最低值相差4.17×105倍, EC90值的最高值与最低值相差7.49×106倍。建议在热带亚热带水果病害防治中,慎用多菌灵等苯并咪唑类杀菌剂,选择作用机制不同的杀菌剂交替使用,延缓抗药性的产生,提高杀菌剂的防效。
採用生長速率法對來自海南18種熱帶亞熱帶水果的893株膠孢炭疽菌(C. gloeosporioides)進行多菌靈抗藥性測定。結果錶明:在供試菌株中,11.87%的菌株對多菌靈產生瞭不同程度的抗性,且高抗菌株達9.63%,具有抗多菌靈的 C. gloeosporioides 菌株主要來自芒果、荔枝和龍眼等14種水果。多菌靈對供試菌株的毒力存在差異, EC50值的最高值與最低值相差4.17×105倍, EC90值的最高值與最低值相差7.49×106倍。建議在熱帶亞熱帶水果病害防治中,慎用多菌靈等苯併咪唑類殺菌劑,選擇作用機製不同的殺菌劑交替使用,延緩抗藥性的產生,提高殺菌劑的防效。
채용생장속솔법대래자해남18충열대아열대수과적893주효포탄저균(C. gloeosporioides)진행다균령항약성측정。결과표명:재공시균주중,11.87%적균주대다균령산생료불동정도적항성,차고항균주체9.63%,구유항다균령적 C. gloeosporioides 균주주요래자망과、려지화용안등14충수과。다균령대공시균주적독력존재차이, EC50치적최고치여최저치상차4.17×105배, EC90치적최고치여최저치상차7.49×106배。건의재열대아열대수과병해방치중,신용다균령등분병미서류살균제,선택작용궤제불동적살균제교체사용,연완항약성적산생,제고살균제적방효。
893 isolates of Colletotrichum gloeosporioides collected from 18 tropical and subtropical fruits in Hainan Province were characterized for resistance to carbendazim by mycelia growth method. The results showed that the isolates had serious resistance to carbendazim with resistance frequency of 11.87%, and 9.63% isolates belonged to high level resistance. The EC50 value and EC90 value of test isolates ranged from 0.03 μg/mL to 12 511.80 μg/mL and 0.15 μg/mL to 1 123 446.50 μg/mL, and the EC50max/EC50min and EC90max/EC90 min reached 4.17×105 and 7.49×106. The resistant isolates were collected from 14 fruits such as mango, litchi and longan. To avoid the resistance of the pathogen to fungicides, and increase the control effect, Benzimidazole fungicides should be cautiously used and different mechanism's fungicides should be used by turns.