CAJ | 학술논문

目的：探讨DNA甲基转移酶1（DNMT1）、程序性细胞死亡因子4（PDCD4）在舌鳞状细胞癌（TSCC）组织中的表达及临床意义。方法采用免疫组织化学 Elivision 二步法检测40例 TSCC 组织及其相对应的癌旁非肿瘤组织中 DNM T1、PDCD4蛋白表达水平，并分析两者蛋白表达与临床病理参数的关系。结果在 TSCC 组织中 DNM T1蛋白呈高表达，而 PDCD4蛋白呈低表达甚至不表达；两者在癌旁非肿瘤组织中的表达则与之相反。两者的表达呈明显的负相关（r ＝－0．452，P ＜0．05）。 DN‐M T1蛋白表达与组织分化类型、有无淋巴结转移及 TNM 临床分期相关（P＜0．05），与性别、年龄无关；而 PDCD4蛋白的表达则只与区域淋巴结有无转移和 TNM 分期有关，与性别、年龄及组织病理分化程度无关。结论提示 DNM T1、PDCD4蛋白异常表达与 TSCC 的发生、发展密切相关，DNM T1可能通过负向调节 PDCD4的蛋白表达使抑癌基因沉默或消失而致肿瘤发生。
목적：탐토DNA갑기전이매1（DNMT1）、정서성세포사망인자4（PDCD4）재설린상세포암（TSCC）조직중적표체급림상의의。방법채용면역조직화학 Elivision 이보법검측40례 TSCC 조직급기상대응적암방비종류조직중 DNM T1、PDCD4단백표체수평，병분석량자단백표체여림상병리삼수적관계。결과재 TSCC 조직중 DNM T1단백정고표체，이 PDCD4단백정저표체심지불표체；량자재암방비종류조직중적표체칙여지상반。량자적표체정명현적부상관（r ＝－0．452，P ＜0．05）。 DN‐M T1단백표체여조직분화류형、유무림파결전이급 TNM 림상분기상관（P＜0．05），여성별、년령무관；이 PDCD4단백적표체칙지여구역림파결유무전이화 TNM 분기유관，여성별、년령급조직병리분화정도무관。결론제시 DNM T1、PDCD4단백이상표체여 TSCC 적발생、발전밀절상관，DNM T1가능통과부향조절 PDCD4적단백표체사억암기인침묵혹소실이치종류발생。
Objective To investigate the expression of DNM T1 and PDCD4 in Tongue Squamous cell carcinoma and their signif‐icance to Clinical .Methods The protein expression of DNM T1 and PDCD4 in 40 cases of TSCC tissues and The adjacent no tumor tissues were measured by Elivision Two Step immunohistochemical method(IHC) ,the relationship between DNM T1 ,PDCD4 and clinicopathological parameters were analyzed .Results There was positively over expression of DNM T1 while the expression of PD‐CD4 was at a low lever or lost in TSCC tissues ,the expression of two genes DNM T1 and PDCD4 in the adjacent no tumor tissues were in contrast ;there was a significant negative correlation between DNM T1 and PDCD4 expression in TSCC(r = - 0 .452 ,P<0 .05) .The expression of DNM T1 protein were associated with histopathological differentiation types ,regional lymph node metasta‐sis and TNM staging(P< 0 .05) ,it had nothing to do with age and gender ;the expression of PDCD4 protein were associated with regional lymph node metastasis and TNM staging(P< 0 .05) ,and it had nothing to do with age ,gender ,histopathological differenti‐ation types .Conclusion Implying the abnormal expression of DNM T1 and PDCD4 protein might be closely related to the develop‐ment and progression of TSCC .DNM T1 may be participates in the inactivation of PDCD4 expression ,and led to the development of the cancer at last .