CAJ | 학술논문

目的：研究S100A4 siRNA对关节炎大鼠炎症及细胞因子肿瘤坏死因子α（TNF‐α）、IL‐1β和血管内皮生长因子（VEGF）表达的影响。方法建立大鼠佐剂性关节炎模型，造模后第11天给模型干扰组大鼠关节腔内注射 S100A4 siRNA 片段。观察各组大鼠关节炎指数（AI）的变化及踝关节的病理变化；ELISA 检测血清 TNF‐α、IL‐1β和 VEGF 的表达变化。结果模型干扰组大鼠 TNF‐α、IL‐1β和 VEGF 表达水平均比模型组降低（P＜0．05）；AI 值及踝关节病理积分与模型组比较显著下降，差异有统计学意义（P＜0．05）。结论抑制 S100A4基因表达，能显著降低致炎细胞因子 TNF‐α、IL‐1β及促血管生成因子 VEGF 的表达，减轻关节滑膜的病理损伤。
목적：연구S100A4 siRNA대관절염대서염증급세포인자종류배사인자α（TNF‐α）、IL‐1β화혈관내피생장인자（VEGF）표체적영향。방법건립대서좌제성관절염모형，조모후제11천급모형간우조대서관절강내주사 S100A4 siRNA 편단。관찰각조대서관절염지수（AI）적변화급과관절적병리변화；ELISA 검측혈청 TNF‐α、IL‐1β화 VEGF 적표체변화。결과모형간우조대서 TNF‐α、IL‐1β화 VEGF 표체수평균비모형조강저（P＜0．05）；AI 치급과관절병리적분여모형조비교현저하강，차이유통계학의의（P＜0．05）。결론억제 S100A4기인표체，능현저강저치염세포인자 TNF‐α、IL‐1β급촉혈관생성인자 VEGF 적표체，감경관절활막적병리손상。
Objective To observe the effects of S100A4 siRNA on the expression of serum TNF‐α,IL‐1βand VEGF in adjuvant arthritis rats .Methods Adjuvant arthritis rat models were established and were randomly divided into model group and interfere group .On Day 11 ,rats in interfere group were injected with S100A4 siRNA fragment in articular cavity .Arthritis index (AI) chan‐ges and pathological changes of ankle joint were observed .The levels of serum TNF‐α and IL‐1β ,VEGF were detected by ELISA . Results Compared with that of model group ,the levels of serum TNF‐ α ,IL‐1β and VEGF were reduced significantly in interfere group (P< 0 .05) ;variances of AI and pathological scores in interfere group were diminished significantly (P< 0 .05) .Conclusion Inhibition of the expression of S100A4 gene can significantly reduce the expression of inflammatory factor TNF‐α ,IL‐1 β and angio‐genesis factor VEGF ,and improve the pathological injury of synovial membrane .