CAJ | 학술논문

目的：探讨白芍总苷（TGP）辅助治疗系统性红斑狼疮（SLE）对患者外周血CD4＋CD25＋T细胞表达水平的影响。方法采用流式细胞术检测健康对照组、常规组、TGP组外周血CD4＋CD25＋T细胞比率。结果SLE患者CD4＋CD25＋T细胞表达率为（6．15±1．21）％，健康对照组CD4＋CD25＋T细胞表达率为（12．30±1．78）％。活动期SLE患者与健康对照组CD4＋CD25＋T细胞表达率比较，差异有统计学意义（t＝22．03，P＜0．05）；常规组治疗前后外周血CD4＋CD25＋T细胞的表达水平差异有统计学意义（t＝－12．30，P＜0．05）；TGP组治疗前后外周血CD4＋CD25＋T细胞的表达水平差异也有统计学意义（t＝－16．68，P＜0．05）。常规组外周血CD4＋CD25＋T细胞的表达水平为（9．34±1．37）％，TGP组外周血CD4＋CD25＋T细胞的表达水平为（11．49±1．14）％，两组差异有统计学意义（t＝－6．46，P＜0．05）。结论活动期SLE患者CD4＋CD25＋T细胞表达率水平显著低于健康对照组；SLE患者经过TGP辅助治疗后CD4＋CD25＋T细胞表达率显著提高。TGP治疗可能作用于CD4＋CD25＋T细胞。
목적：탐토백작총감（TGP）보조치료계통성홍반랑창（SLE）대환자외주혈CD4＋CD25＋T세포표체수평적영향。방법채용류식세포술검측건강대조조、상규조、TGP조외주혈CD4＋CD25＋T세포비솔。결과SLE환자CD4＋CD25＋T세포표체솔위（6．15±1．21）％，건강대조조CD4＋CD25＋T세포표체솔위（12．30±1．78）％。활동기SLE환자여건강대조조CD4＋CD25＋T세포표체솔비교，차이유통계학의의（t＝22．03，P＜0．05）；상규조치료전후외주혈CD4＋CD25＋T세포적표체수평차이유통계학의의（t＝－12．30，P＜0．05）；TGP조치료전후외주혈CD4＋CD25＋T세포적표체수평차이야유통계학의의（t＝－16．68，P＜0．05）。상규조외주혈CD4＋CD25＋T세포적표체수평위（9．34±1．37）％，TGP조외주혈CD4＋CD25＋T세포적표체수평위（11．49±1．14）％，량조차이유통계학의의（t＝－6．46，P＜0．05）。결론활동기SLE환자CD4＋CD25＋T세포표체솔수평현저저우건강대조조；SLE환자경과TGP보조치료후CD4＋CD25＋T세포표체솔현저제고。TGP치료가능작용우CD4＋CD25＋T세포。
Objective to investigate the effect of TGP assisted treatment of SLE on the expression of CD4+CD25+ T in patient peripheral blood .Methods flow cytometry was used to detect the peripheral blood CD4+ CD25+ T cells in healthy group ,routine group and TGP group .Results The expression rate of CD4+CD25+ T cells in SLE patients was (6 .15 ± 1 .21)% ,and that of the healthy controls was (12 .30 ± 1 .78)% .The expression rate of CD4+CD25+ T cells in active SLE patients and healthy controls were significantly different (t=22 .03 ,P<0 .05) .In the routine group ,the expression rate of peripheral blood CD4+CD25+ T cells before and after treatment were significantly different (t= 12 .30 ,P<0 .05);in the TGP group ,the expression rate of peripheral blood CD4+ CD25+ T cells before and after treatment were significantly different ,too (t=16 .68 ,P<0 .05) .The expression rate of periph‐eral blood CD4+CD25+ T cells in routine group and TGP group were (9 .34 ± 1 .37)% and (11 .49 ± 1 .14)% respectively ,and the difference was statistically significant (t=6 .46 ,P<0 .05) .Conclusion The expression rate of CD4+ CD25+ T cells in active SLE patients was significantly lower than that of healthy controls ;the expression rate of CD4+CD25+ T cells in SLE patients increased significantly after treatment .The TGP treatment may work on CD4+CD25+ T cells .