CAJ | 학술논문

目的：探讨大蒜素对实验性贝赫切特病（BD）的治疗作用及机制。方法构建BD模型鼠，将其分为实验组、阳性对照组和阴性对照组，分别鼻饲注入大蒜素、秋水仙碱和生理盐水。肉眼观察治疗前后皮损的变化，ELISA法检测治疗前后血清γ干扰素（IFN‐γ）、肿瘤坏死因子α（TNF‐α）、IL‐4和化学比色法检测总抗氧化力（T‐AOC）、丙二醛（MDA）、超氧化物歧化酶（SOD）、谷甘肽过氧化物酶（GSH‐PX）。结果模型小鼠背、腹、阴部出现溃疡，IFN‐γ、TNF‐α、IL‐4和MDA升高，T‐AOC、SOD和GSH‐PX下降。治疗20d后实验组和阳性对照组小鼠溃疡基本愈合；30d后实验组仅2只复发，阳性对照组有6只复发，差异有统计学意义（P＜0．05）。治疗后实验组IFN‐γ、TNF‐α和IL‐4较治疗前降低（P＜0．01），与阳性对照组比较差异无统计学意义（P＞0．05）；实验组T‐AOC、SOD和GSH‐PX较治疗前升高，MDA下降（P＜0．01），与阳性对照组比较差异有统计学意义（P＜0．05）。结论大蒜素是治疗实验性BD的有效药物，可能是通过抑制炎症因子、阻止氧化应激而作用。
목적：탐토대산소대실험성패혁절특병（BD）적치료작용급궤제。방법구건BD모형서，장기분위실험조、양성대조조화음성대조조，분별비사주입대산소、추수선감화생리염수。육안관찰치료전후피손적변화，ELISA법검측치료전후혈청γ간우소（IFN‐γ）、종류배사인자α（TNF‐α）、IL‐4화화학비색법검측총항양화력（T‐AOC）、병이철（MDA）、초양화물기화매（SOD）、곡감태과양화물매（GSH‐PX）。결과모형소서배、복、음부출현궤양，IFN‐γ、TNF‐α、IL‐4화MDA승고，T‐AOC、SOD화GSH‐PX하강。치료20d후실험조화양성대조조소서궤양기본유합；30d후실험조부2지복발，양성대조조유6지복발，차이유통계학의의（P＜0．05）。치료후실험조IFN‐γ、TNF‐α화IL‐4교치료전강저（P＜0．01），여양성대조조비교차이무통계학의의（P＞0．05）；실험조T‐AOC、SOD화GSH‐PX교치료전승고，MDA하강（P＜0．01），여양성대조조비교차이유통계학의의（P＜0．05）。결론대산소시치료실험성BD적유효약물，가능시통과억제염증인자、조지양화응격이작용。
Objective To explore the therapeutic effect and the mechanism of allicin on experimental Behcet′s disease (BD) . Methods BD models were established .Then ,model mice were randomly divided into experimental group ,positive control group and negative control group ,with respectively nasogastric infusion allicin ,colchicine and saline respectively .Before and after treatment , the changes of the skin lesions were visually observed .Cytokines of IFN‐γ,TNF‐αand IL‐4 in serum were detected by ELISA anal‐ysis ,while the markers of T‐AOC ,MDA ,SOD and GSH‐PX by chemical colorimetric methods .Results After modeling ,ulcers ap‐peared on the back ,abdomen and genital of model mice with IFN‐γ,TNF‐α,IL‐4 and MDA elevation ,while T‐AOC ,SOD and GSH‐PX decrease .Twenty days of treatment later ,ulcer on mice in the experimental group and positive control group healed .After 30 days ,only two mice in the experimental group recurred ,while 6 mice in the positive control group ,which showed a significant differ‐ence between two groups (P<0 .05) .After treatment ,IFN‐γ,TNF‐αand IL‐4 levels in the experimental group were markedly low‐er than pretreatment (P<0 .01) ,but no significant difference compared with the positive control group (P>0 .05) .Meanwhile ,T‐AOC ,SOD and GSH‐PX levels in the experimental group were significantly higher than those in the pretreatment ,but MDA was lower (P<0 .01) .In comparison with the positive control group ,levels of those markers in the experimental group changed remark‐ably (P<0 .05) .Conclusion Allicin is an effective drug in the treatment of experimental BD .The therapeutic mechanism of allicin may be the inhibition of inflammatory mediators and prevention of oxidative stress .