重庆医学
重慶醫學
중경의학
CHONGQING MEDICAL JOURNAL
2014年
33期
4500-4502
,共3页
万涛%黄习臣%粟毅%李岱容
萬濤%黃習臣%粟毅%李岱容
만도%황습신%속의%리대용
纤维支气管镜抽吸物%液基细胞制片%细胞学诊断
纖維支氣管鏡抽吸物%液基細胞製片%細胞學診斷
섬유지기관경추흡물%액기세포제편%세포학진단
fiber bronchoscopic aspirates%liquid based cytology test%cytological diagnosis
目的：探讨液基细胞制片技术在纤维支气管镜抽吸物检测中的应用价值。方法采用安必平（LBP）沉降式液基细胞制片系统并结合传统涂片方法处理纤维支气管镜抽吸标本2000例，制成薄片，显微镜下观察，进行细胞学诊断。结果液基细胞制片法共检出细胞学阳性病例614例（30．7％），其中鳞癌267例（13．4％），腺癌217例（10．9％），小细胞癌83例（4．2％），未定型癌47例（2．4％）。液基制片法诊断肺癌敏感度为64．6％，传统涂片法诊断肺癌敏感度为49．2％。同时发现真菌阳性7例，结核病典型的干酪样坏死35例。结论液基细胞制片比传统方法制备的涂片背景更干净，细胞结构保持完整，阅片效率更高，癌细胞检出的阳性率得到提高，两种方法同时采用能最大限度提高纤维支气管镜细胞学检测的准确性和阳性检出率，同时还能为真菌、结核菌感染提供诊断依据。
目的：探討液基細胞製片技術在纖維支氣管鏡抽吸物檢測中的應用價值。方法採用安必平（LBP）沉降式液基細胞製片繫統併結閤傳統塗片方法處理纖維支氣管鏡抽吸標本2000例，製成薄片，顯微鏡下觀察，進行細胞學診斷。結果液基細胞製片法共檢齣細胞學暘性病例614例（30．7％），其中鱗癌267例（13．4％），腺癌217例（10．9％），小細胞癌83例（4．2％），未定型癌47例（2．4％）。液基製片法診斷肺癌敏感度為64．6％，傳統塗片法診斷肺癌敏感度為49．2％。同時髮現真菌暘性7例，結覈病典型的榦酪樣壞死35例。結論液基細胞製片比傳統方法製備的塗片揹景更榦淨，細胞結構保持完整，閱片效率更高，癌細胞檢齣的暘性率得到提高，兩種方法同時採用能最大限度提高纖維支氣管鏡細胞學檢測的準確性和暘性檢齣率，同時還能為真菌、結覈菌感染提供診斷依據。
목적：탐토액기세포제편기술재섬유지기관경추흡물검측중적응용개치。방법채용안필평（LBP）침강식액기세포제편계통병결합전통도편방법처리섬유지기관경추흡표본2000례，제성박편，현미경하관찰，진행세포학진단。결과액기세포제편법공검출세포학양성병례614례（30．7％），기중린암267례（13．4％），선암217례（10．9％），소세포암83례（4．2％），미정형암47례（2．4％）。액기제편법진단폐암민감도위64．6％，전통도편법진단폐암민감도위49．2％。동시발현진균양성7례，결핵병전형적간락양배사35례。결론액기세포제편비전통방법제비적도편배경경간정，세포결구보지완정，열편효솔경고，암세포검출적양성솔득도제고，량충방법동시채용능최대한도제고섬유지기관경세포학검측적준학성화양성검출솔，동시환능위진균、결핵균감염제공진단의거。
Objective To study liquid based cytology test and its application values in the detection of fiber bronchoscopic aspi‐rates .Methods LBP sedimentation type of ThinPrep cell production system combined with the conventional smear were used to de‐tect 2000 specimens of fiber bronchoscopic aspirates ,and conduct microscope observation and cytological diagnosis .Results Based on liquid based cytology cell production method ,there were 614 positive cases (30 .7% ) ,267 cases of squamous cell carcinoma (13 .4% ) ,and 217 cases of adenocarcinoma(10 .9% ) ,83 cases of small cell carcinoma (4 .2% ) ,and 47 cases of undifferentiated car‐cinoma (2 .4% ) .The diagnostic sensitivity for lung cancer of liquid‐based cytology test was 64 .6% and conventional smear was 49 .2% .We also found 7 cases of fungus positive ,35 cases of typical caseous necrosis with liquid based cytology test .Conclusion The ThinPrep cell production has a more clean background ,more intact cell structure ,more efficient observation and increased posi‐tive rate of detection of cancer cells than the smears made by traditional methods .A combination of two methods can increase the accuracy of cytological detection of fiber bronchoscope aspirates and positive detection rate ,and also can assist the diagnosis of fungi and TB infections .
