CAJ | 학술논문

目的：探索油酸（OA）诱导建立人正常肝细胞Changliver脂肪变性的离体细胞模型的实验条件。方法选用不同浓度油酸作用不同时间点诱导Changliver细胞，并用四甲基偶氮唑盐比色（MTT）法检测细胞活性，油红O染色观察细胞内脂滴数量、甘油‐3‐磷酸氧化酶法检测细胞内三酰甘油（TG）含量。结果采用0．2mmol/LOA诱导24h能建立较好的Changliver细胞脂肪变性模型，模型组细胞内TG水平为（379．98±23．19）mg/g，空白组细胞内TG水平为（185．03±12．68）mg/g，两组比较差异有统计意义（P＜0．01）。结论0．2mmol/LOA油酸诱导的Changliver细胞24h可建立比较稳定的脂肪变性模型。该模型为非酒精性脂肪性肝病的研究提供可靠的新途径。
목적：탐색유산（OA）유도건립인정상간세포Changliver지방변성적리체세포모형적실험조건。방법선용불동농도유산작용불동시간점유도Changliver세포，병용사갑기우담서염비색（MTT）법검측세포활성，유홍O염색관찰세포내지적수량、감유‐3‐린산양화매법검측세포내삼선감유（TG）함량。결과채용0．2mmol/LOA유도24h능건립교호적Changliver세포지방변성모형，모형조세포내TG수평위（379．98±23．19）mg/g，공백조세포내TG수평위（185．03±12．68）mg/g，량조비교차이유통계의의（P＜0．01）。결론0．2mmol/LOA유산유도적Changliver세포24h가건립비교은정적지방변성모형。해모형위비주정성지방성간병적연구제공가고적신도경。
Objective To explore the experimental condition for hepatocellular steatosis models of Changliver cell induced by o‐leic acid (lleic acid ,OA) .Methods Changliver cells were induced by different concentration of oleic acid for different periods .MTT was used to detect hepatic cell activity ,oil red 0 staining was used to observe intracellular lipid droplets acumulation ,glycerin 3 phosphate oxidase method was applied to detect the contents of triglyceride (TG) in the Changliver cell .Results Hepatocellular steatosis models of Changliver cell can be established successfully by 0 .2 mmol/L OA inducing for 24 hours .TG content in model cells was (379 .98 ± 23 .19)mg/g ,however ,it was (185 .03 ± 12 .68)mg/g in control cells ,the difference was statistically significant (P< 0 .01) .Conclusion The proper condition for establishing hepatocellular steatosis models is 0 .2 mmol/L OA inducing Changliver cells for 24 h .This model is the reliable choice for nonalcoholic fatty liver disease research .