CAJ | 학술논문

目的：探讨辛伐他汀治疗大鼠实验性肺动脉高压（PH）的疗效及其对循环内皮祖细胞（EPC）数量和功能的影响。方法选取Sprague‐Dawley大鼠24只，随机分为3组：模型组、干预组、对照组，每组8只。利用野百合碱皮下注射制备PH模型，干预组大鼠于第3天应用辛伐他汀对其进行治疗，第21天测定比较各组大鼠右室收缩压（RVSP）、肺血管结构和循环 EPC的数量，同时体外培养各组EPC ，对各组EPC的产量和功能进行分析比较。结果干预组循环EPC含量明显高于模型组及对照组（P＜0．01）；和模型组比较，干预组RVSP、肺小动脉中膜厚度与血管外径比值显著降低（P＜0．01），管腔面积与血管总面积比值（VA/TAA）显著增高（P＜0．01）；体外培养下，干预组EPCs的产量以及增殖、黏附、迁移能力均较模型组显著增高。结论辛伐他汀能够通过有效上调PH模型大鼠循环EPC的数量及功能，从而达到对PH的治疗目的。
목적：탐토신벌타정치료대서실험성폐동맥고압（PH）적료효급기대순배내피조세포（EPC）수량화공능적영향。방법선취Sprague‐Dawley대서24지，수궤분위3조：모형조、간예조、대조조，매조8지。이용야백합감피하주사제비PH모형，간예조대서우제3천응용신벌타정대기진행치료，제21천측정비교각조대서우실수축압（RVSP）、폐혈관결구화순배 EPC적수량，동시체외배양각조EPC ，대각조EPC적산량화공능진행분석비교。결과간예조순배EPC함량명현고우모형조급대조조（P＜0．01）；화모형조비교，간예조RVSP、폐소동맥중막후도여혈관외경비치현저강저（P＜0．01），관강면적여혈관총면적비치（VA/TAA）현저증고（P＜0．01）；체외배양하，간예조EPCs적산량이급증식、점부、천이능력균교모형조현저증고。결론신벌타정능구통과유효상조PH모형대서순배EPC적수량급공능，종이체도대PH적치료목적。
Objective The present study was designed to investigate the efficiency of simvastatin therapy for experimental pul‐monary hypertension (PH) in rat ,and the effects on the number and function of circulating endothelial progenitor cells (EPC) . Methods Twenty four Sprague Dawley rats were divided into 3 groups randomly :model group ,treatment group and control group , 8 rats in each group .Rats were treated with a single subcutaneous injection of monocrotaline to induce PH (PBS used as control) . The rats in the experimental group were administrated with simvastatin 3 days following subcutaneous injection of monocrotaline .In the 21st day ,the number of circulating EPC ,the right ventricle systolic pressure of rat ,pulmonary vascular structural changes and the quantity of cultured EPC were measured .At the same time ,EPC in each group were cultured in vitro ,then the ability of prolif‐eration and function were analyzed and compared .Results The number of circulating EPC in model group was decreased signifi‐cantly compared to both control and model groups (P< 0 .01) .Compared with model group ,simvastatin treatment markedly de‐creased the RVSP and the ratio of media thickness to eternal diameter (P<0 .01) ,but the ratio of vessel area to total arterial area (VA/TAA) was definitively increased(P<0 .01) .After 7 days of culture in vitro ,both the output of EPC and the ability of prolif‐eration ,conglutination and migration of EPC in treatment group were up -regulated compared with those in model group (P<0 .01) .Conclusion This study confirmed that simvastatin effectively treat experimental PH through improving quantity and func‐tion of circulating EPC .