CAJ | 학술논문

目的：通过比较体外循环机持续灌注、间断压力灌注、单存低温保存离体肺的水通道蛋白1（AQP1）表达变化，探索离体肺保存的最佳保存方法。方法将30只Mongrel犬分成体外循环组、压力灌注组、低温保存组，每组10只。在保持机械通气的条件下，完整摘取双肺。进行体外循环机灌注、间断压力灌注、单存灌注离体肺循环后低温浸泡保存，并按时间点留取标本；HE染色法观察犬离体肺组织形态学改变，免疫组织化学法、蛋白免疫印迹法（Westernblot）检测犬离体肺组织AQP1的表达。结果HE染色发现，随着离体时间的延长，肺泡组织结构逐渐塌陷，炎性细胞及渗出物增多，肺泡间隔也逐渐增宽，肺泡腔内可见渗出物；在同一时间点，体外循环组的组织结构损伤变化比压力灌注组和低温保存组轻。在各实验组随着时间推移AQP1的表达量呈现下降趋势；在每个时间点上，体外循环组AQP1的表达量高于压力灌注组，压力灌注组又高于低温保存组。结论体外循环机械灌注对离体肺的保护作用显著优于间断压力灌注和单存低温保存。
목적：통과비교체외순배궤지속관주、간단압력관주、단존저온보존리체폐적수통도단백1（AQP1）표체변화，탐색리체폐보존적최가보존방법。방법장30지Mongrel견분성체외순배조、압력관주조、저온보존조，매조10지。재보지궤계통기적조건하，완정적취쌍폐。진행체외순배궤관주、간단압력관주、단존관주리체폐순배후저온침포보존，병안시간점류취표본；HE염색법관찰견리체폐조직형태학개변，면역조직화학법、단백면역인적법（Westernblot）검측견리체폐조직AQP1적표체。결과HE염색발현，수착리체시간적연장，폐포조직결구축점탑함，염성세포급삼출물증다，폐포간격야축점증관，폐포강내가견삼출물；재동일시간점，체외순배조적조직결구손상변화비압력관주조화저온보존조경。재각실험조수착시간추이AQP1적표체량정현하강추세；재매개시간점상，체외순배조AQP1적표체량고우압력관주조，압력관주조우고우저온보존조。결론체외순배궤계관주대리체폐적보호작용현저우우간단압력관주화단존저온보존。
Objective By comparing the change of expression of AQP1 in vitro lung preserved by the continuous infusion of a heart‐lung machine ,continuous pressure perfusion and single low temperature ,to explore the best method of vitro lung preserva‐tion .Methods Thirty Mongrel dogs were randomly divided into 3 groups ,and both lungs were completely resected under the condi‐tion of keeping mechanical ventilation .The vitro lungs were preserved by the way of the continuous infusion of a heart‐lung ma‐chine ,continuous pressure perfusion and single low temperature ,and collecting specimens according to the time point .HE staining was used to observe the morphological changes of vitro lung tissue .Immunohistochemistry and Western blot were used to detect the expression of AQP1 in vitro lung .Results HE staining found that as the time went by alveolar structure gradually collapsed ,in‐flammatory cells increased ,alveolar interval also gradually broadened and exudation could be seen in the alveolar cavity ;at the same time point ,organization structure of extracorporeal circulation group changed lighter than pressure perfusion group and low‐temper‐ature preservation group .In each experimental group ,the expression of AQP1 showed a trend of decline;at each time point ,the ex‐pression of AQP1 in extracorporeal circulation group was higher than pressure perfusion group ,and pressure infusion group was higher than that of low‐temperature preservation group .Conclusion The protective effect of the continuous infusion of a heart‐lung machine on vitro lung was better than continuous pressure perfusion and single low temperature .