CAJ | 학술논문

目的：探讨Ion Torrent测序检测非小细胞肺癌外周血DNA的临床价值。方法：选择患者80例，分为两组，各40例，观察组利用三代测序技术 Ion Torrent检测，对照组使用第1代测序技术Sanger末端终止法进行测序，比较手术前后通过Ion Torrent测序所得EGFR和K－ras结果，并统计两组测序EGFR阳性1年生存情况。结果：未手术者观察组EGFR阳性和K－ras突变阳性检出率均显著高于对照组（P＜0．05），术后复发患者观察组EGFR阳性和K－ras突变阳性检出率均显著高于对照组（P＜0．05），观察组1年生存率显著高于对照组（P＜0．05）。结论：对非小细胞肺癌患者使用Ion Torrent测序技术测定外周血DNA，能更准确的确定患者基因类型，从而为分子靶向治疗提供依据。
목적：탐토Ion Torrent측서검측비소세포폐암외주혈DNA적림상개치。방법：선택환자80례，분위량조，각40례，관찰조이용삼대측서기술 Ion Torrent검측，대조조사용제1대측서기술Sanger말단종지법진행측서，비교수술전후통과Ion Torrent측서소득EGFR화K－ras결과，병통계량조측서EGFR양성1년생존정황。결과：미수술자관찰조EGFR양성화K－ras돌변양성검출솔균현저고우대조조（P＜0．05），술후복발환자관찰조EGFR양성화K－ras돌변양성검출솔균현저고우대조조（P＜0．05），관찰조1년생존솔현저고우대조조（P＜0．05）。결론：대비소세포폐암환자사용Ion Torrent측서기술측정외주혈DNA，능경준학적학정환자기인류형，종이위분자파향치료제공의거。
Objective:To investigate the clinical value of ion torrent sequencing with DNA for non -small cell peripheral lung cancer .Method:80 cases were divided into two groups , each with 40 cases, the observation group were used three generations with ion torrent sequencing technology ,the control group were used the first-generation sequencing technology method with Sanger sequencing , then sequencing proceeds before and after surgery and EGFR by ion torrent K-ras results and statistics sequencing EGFR-positive a two-year survival were compared .Result:The observation group with no surgery of EGFR-positive and K-ras mutation positive detection rate were significantly higher than those of control group (P<0.05), the ob-servation group with recurrence of EGFR-positive group and K-ras mutations were significantly higher than those of control group ( P<0.05) , 1-year survival rate in the observation group was significantly higher than that of control group (P<0.05).Conclusion:Ion torrent sequencing technology to measure peripheral blood DNA with non-small cell lung cancer patients can more accurately determine the genetic type of the patient , and thus provide the basis for molecular targeted therapy .