新疆大学学报(自然科学版)
新疆大學學報(自然科學版)
신강대학학보(자연과학판)
XINJIANG UNIVERSITY JOURNAL(NATURAL SCIENCE EDITION)
2014年
4期
481-486
,共6页
祖克拉·肉孜%吐尔逊·买买提%宋曼殳%多力坤·买买提玉素甫
祖剋拉·肉孜%吐爾遜·買買提%宋曼殳%多力坤·買買提玉素甫
조극랍·육자%토이손·매매제%송만수%다력곤·매매제옥소보
2型糖尿病%糖耐量%血管紧张素转换酶%基因多态性%新疆%两个民族
2型糖尿病%糖耐量%血管緊張素轉換酶%基因多態性%新疆%兩箇民族
2형당뇨병%당내량%혈관긴장소전환매%기인다태성%신강%량개민족
type 2 diabetes%glucose tolerance%ACE gene polymorphism%Xinjiang minority people
探讨新疆柯尔克孜族和维吾尔族人群血管紧张素转化酶(ACE)基因rs1799752位点插入或缺失(I/D)多态性及其与2型糖尿病(TypeⅡdiabetes mellitus,T2DM)的关系.方法采用病例-对照的研究设计.采集无血缘关系、年龄性别匹配的新疆柯尔克孜族样本117例、维吾尔族样本127例,分为2型糖尿病组(T2DM)、糖耐量异常组(impaired glucose tolerance, IGT)和糖耐量正常组(normal glucose tolerance, NGT),以Hardy-Weinberg平衡检验确认研究样本的群体代表性,采用PCR技术检测ACE基因I/D多态性.结果(1)病例组与对照组ACE基因多态性的分布符合Hardy-Weinberg平衡定律,所选人群具有代表性;(2)柯尔克孜族DD型42.74%,ID型31.58%,II型26.50%,D和I 等位基因频率分别为58.115%和41.876%;维吾尔族DD型35.43%,ID型28.35%,II型36.22%,D和I 等位基因频率分别为49.626%和50.387%.(3)ACE基因频率差异在柯尔克孜族(χ2=70.11,P <0.01),维吾尔族(χ2=35.11,P<0.01)的糖调节异常组与糖耐量正常组间,分别具有统计学意义.在维吾尔族T2DM组中携带DD、ID、ID+DD基因型的个体较携带II基因型的个体发生糖代谢异常(T2DM+IGT)的危险性增加(OR=7.812,95%CI=3.135-19.607;OR=4.854,95%CI=1.835-12.821;OR=6.410,95%CI=2.865-14.286,携带D等位基因的个体较携带I等位基因的个体发生糖代谢异常(T2DM+IGT)的危险性增加(OR=4.444,95%CI=2.617-7.576),而柯尔克孜族DD、ID+DD基因型和D等位基因性对糖代谢异常(T2DM+IGT)的发病风险降低.结论ACE基因I/D多态性与新疆两个少数民族T2DM相关,ACE基因I/D多态性可能是维吾尔族T2DM的危险因素,柯尔克孜族的保护因素.
探討新疆柯爾剋孜族和維吾爾族人群血管緊張素轉化酶(ACE)基因rs1799752位點插入或缺失(I/D)多態性及其與2型糖尿病(TypeⅡdiabetes mellitus,T2DM)的關繫.方法採用病例-對照的研究設計.採集無血緣關繫、年齡性彆匹配的新疆柯爾剋孜族樣本117例、維吾爾族樣本127例,分為2型糖尿病組(T2DM)、糖耐量異常組(impaired glucose tolerance, IGT)和糖耐量正常組(normal glucose tolerance, NGT),以Hardy-Weinberg平衡檢驗確認研究樣本的群體代錶性,採用PCR技術檢測ACE基因I/D多態性.結果(1)病例組與對照組ACE基因多態性的分佈符閤Hardy-Weinberg平衡定律,所選人群具有代錶性;(2)柯爾剋孜族DD型42.74%,ID型31.58%,II型26.50%,D和I 等位基因頻率分彆為58.115%和41.876%;維吾爾族DD型35.43%,ID型28.35%,II型36.22%,D和I 等位基因頻率分彆為49.626%和50.387%.(3)ACE基因頻率差異在柯爾剋孜族(χ2=70.11,P <0.01),維吾爾族(χ2=35.11,P<0.01)的糖調節異常組與糖耐量正常組間,分彆具有統計學意義.在維吾爾族T2DM組中攜帶DD、ID、ID+DD基因型的箇體較攜帶II基因型的箇體髮生糖代謝異常(T2DM+IGT)的危險性增加(OR=7.812,95%CI=3.135-19.607;OR=4.854,95%CI=1.835-12.821;OR=6.410,95%CI=2.865-14.286,攜帶D等位基因的箇體較攜帶I等位基因的箇體髮生糖代謝異常(T2DM+IGT)的危險性增加(OR=4.444,95%CI=2.617-7.576),而柯爾剋孜族DD、ID+DD基因型和D等位基因性對糖代謝異常(T2DM+IGT)的髮病風險降低.結論ACE基因I/D多態性與新疆兩箇少數民族T2DM相關,ACE基因I/D多態性可能是維吾爾族T2DM的危險因素,柯爾剋孜族的保護因素.
탐토신강가이극자족화유오이족인군혈관긴장소전화매(ACE)기인rs1799752위점삽입혹결실(I/D)다태성급기여2형당뇨병(TypeⅡdiabetes mellitus,T2DM)적관계.방법채용병례-대조적연구설계.채집무혈연관계、년령성별필배적신강가이극자족양본117례、유오이족양본127례,분위2형당뇨병조(T2DM)、당내량이상조(impaired glucose tolerance, IGT)화당내량정상조(normal glucose tolerance, NGT),이Hardy-Weinberg평형검험학인연구양본적군체대표성,채용PCR기술검측ACE기인I/D다태성.결과(1)병례조여대조조ACE기인다태성적분포부합Hardy-Weinberg평형정률,소선인군구유대표성;(2)가이극자족DD형42.74%,ID형31.58%,II형26.50%,D화I 등위기인빈솔분별위58.115%화41.876%;유오이족DD형35.43%,ID형28.35%,II형36.22%,D화I 등위기인빈솔분별위49.626%화50.387%.(3)ACE기인빈솔차이재가이극자족(χ2=70.11,P <0.01),유오이족(χ2=35.11,P<0.01)적당조절이상조여당내량정상조간,분별구유통계학의의.재유오이족T2DM조중휴대DD、ID、ID+DD기인형적개체교휴대II기인형적개체발생당대사이상(T2DM+IGT)적위험성증가(OR=7.812,95%CI=3.135-19.607;OR=4.854,95%CI=1.835-12.821;OR=6.410,95%CI=2.865-14.286,휴대D등위기인적개체교휴대I등위기인적개체발생당대사이상(T2DM+IGT)적위험성증가(OR=4.444,95%CI=2.617-7.576),이가이극자족DD、ID+DD기인형화D등위기인성대당대사이상(T2DM+IGT)적발병풍험강저.결론ACE기인I/D다태성여신강량개소수민족T2DM상관,ACE기인I/D다태성가능시유오이족T2DM적위험인소,가이극자족적보호인소.
Objective To identify the association between ACE I/D polymorphism and genetic susceptibil-ity of type 2 diabetes(T2DM) among two minorities in XinJiang. Methods Case-control design was applied. Gender-matched and age-matched random samples of the two minorities was recruited from separate hospitals and grouped into normal glucose tolerance (NGT), impaired glucose tolerance(IGT) and T2DM subgroups. The genotypes of ACE I/D polymorphism were detected by tolerance(IGT) and T2DM subgroups. The genotypes of ACE I/D polymorphism were detected by polymerase chain reaction ( PCR) methods. Re-sults The genotypic and allelic frequencies of ACE polymorphism Accord with Hardy-Weinberg equilibrium principle the genotypic frequencies of ACE polymorphism in kirgiz respectively DD 42.74% ,ID 31.58% ,II 26.50%,allelic frequencies of D and I was 58.115%41.876%. There was significant difference of the genotypic and allelic frequencies of ACE polymorphism between Patients (T2DM or IGT) and controls(NGT) group. Respectively,in kirgiz(χ2=70.11,P<0.01)and(χ2=35.11,P<0.01)in uyghur people. Multivariate logistic re-gression analysis indicated that DD increased the risk of T2DM for Uyghur(OR=7.812, 95%CI=3.135-19.607;OR=4.854, 95%CI=1.835-12.821; OR=6.410, 95%CI=2.865-14.286.Conclusion: ACE gene polymorphisms were distributed distinctively in Uyghur, Kirgiz ethnic groups in XinJiang. DD might be risk factor of T2DM for Uyghur ethnic group while be protective factor for Kirgiz.