中国听力语言康复科学杂志
中國聽力語言康複科學雜誌
중국은력어언강복과학잡지
CHINESE SCIENTIFIC JOURNAL OF HEARING AND SPEECH REHABILITATION
2014年
6期
424-428
,共5页
C57BL/6J小鼠%老年性耳聋%NKCC1%Na-K-ATPase%听性脑干反应%实时免疫荧光定量逆转录聚合酶链反应
C57BL/6J小鼠%老年性耳聾%NKCC1%Na-K-ATPase%聽性腦榦反應%實時免疫熒光定量逆轉錄聚閤酶鏈反應
C57BL/6J소서%노년성이롱%NKCC1%Na-K-ATPase%은성뇌간반응%실시면역형광정량역전록취합매련반응
C57BL/6J mice%Presbycusis%NKCC1%Na-K-ATPase%ABR%RT-PCR
目的：通过对C57BL/6J小鼠耳蜗中NKCC1和Na-K-ATPase的年龄相关性表达的研究，分析其与老年性耳聋的关系并进一步探讨其在老年性耳聋发生发展不同阶段中的作用。方法通过听性脑干反应（ABR）分别检测C57BL/6J小鼠在4、24和48周年龄段的听力水平。采用实时免疫荧光定量逆转录聚合酶链反应（RT-PCR）法分别检测NKCC1和Na-K-ATPase mRNA在各年龄段小鼠耳蜗中的表达水平。结果随着C57BL/6J鼠龄的增加，其ABR反应阈值逐渐升高（P<0.05）。RT-PCR显示NKCC1和Na-K-ATPase mRNA在耳蜗的表达水平存在随鼠龄增加逐渐下降的趋势（P＜0.01）。结论 C57BL/6J小鼠的ABR反应阈值随鼠龄增加逐渐增高，具有老年性耳聋的特征；NKCC1和Na-K-ATPase两种通道蛋白随鼠龄的增加逐渐下降，与C57BL/6J小鼠的年龄相关性听力下降密切相关，可能与老年性耳聋后期发展及恶化有关。
目的：通過對C57BL/6J小鼠耳蝸中NKCC1和Na-K-ATPase的年齡相關性錶達的研究，分析其與老年性耳聾的關繫併進一步探討其在老年性耳聾髮生髮展不同階段中的作用。方法通過聽性腦榦反應（ABR）分彆檢測C57BL/6J小鼠在4、24和48週年齡段的聽力水平。採用實時免疫熒光定量逆轉錄聚閤酶鏈反應（RT-PCR）法分彆檢測NKCC1和Na-K-ATPase mRNA在各年齡段小鼠耳蝸中的錶達水平。結果隨著C57BL/6J鼠齡的增加，其ABR反應閾值逐漸升高（P<0.05）。RT-PCR顯示NKCC1和Na-K-ATPase mRNA在耳蝸的錶達水平存在隨鼠齡增加逐漸下降的趨勢（P＜0.01）。結論 C57BL/6J小鼠的ABR反應閾值隨鼠齡增加逐漸增高，具有老年性耳聾的特徵；NKCC1和Na-K-ATPase兩種通道蛋白隨鼠齡的增加逐漸下降，與C57BL/6J小鼠的年齡相關性聽力下降密切相關，可能與老年性耳聾後期髮展及噁化有關。
목적：통과대C57BL/6J소서이와중NKCC1화Na-K-ATPase적년령상관성표체적연구，분석기여노년성이롱적관계병진일보탐토기재노년성이롱발생발전불동계단중적작용。방법통과은성뇌간반응（ABR）분별검측C57BL/6J소서재4、24화48주년령단적은력수평。채용실시면역형광정량역전록취합매련반응（RT-PCR）법분별검측NKCC1화Na-K-ATPase mRNA재각년령단소서이와중적표체수평。결과수착C57BL/6J서령적증가，기ABR반응역치축점승고（P<0.05）。RT-PCR현시NKCC1화Na-K-ATPase mRNA재이와적표체수평존재수서령증가축점하강적추세（P＜0.01）。결론 C57BL/6J소서적ABR반응역치수서령증가축점증고，구유노년성이롱적특정；NKCC1화Na-K-ATPase량충통도단백수서령적증가축점하강，여C57BL/6J소서적년령상관성은력하강밀절상관，가능여노년성이롱후기발전급악화유관。
Objective To investigate the expression of ion transporter NKCC1 and Na-K-ATPase in C57BL/6J mouse cochlea, to analyze its relationship with presbycusis and further explore their roles in the different stages of presbycusis development. Methods The auditory function of C57BL/6J mice was measured by auditory brainstem response (ABR) at the ages of 4, 24, 48 weeks old, respectively. The expression level of NKCC1 mRNA, and Na-K-ATPase mRNA in different age C57BL/6J mice were evaluated by using real-time fluorescent quantitative reverse transcription polymerase chain reaction(RT-PCR) . Results The ABR threshold gradually increased with the increasing age of C57BL/6J mice(P<0.05). The results of RT-PCR showed that the expression level of NKCC1 mRNA and Na-K-ATPase mRNA in the mice cochlea decreased with increasing age(P<0.01). Conclusion The changes of ABR threshold in our experiment showed that C57BL/6J mouse is an ideal model of presbycusis study.The expression levels of NKCC1 mRNA and Na-K-ATPase mRNA in C57BL/6J mice showed a downward trend while α1Na-K-ATPase mRNA increased in 24-week-old mice and then decreased in 48-week-old mice. In conclusion, ion transporter NKCC1 and Na-K-ATPase may have significance in the origin and development of presbycusis,but α1Na-K-ATPase may just be associated with the development of its exacerbation. Ion transporter NKCC1, and Na-K-ATPase are closely associated with the age-related hearing loss in C57BL/6J mice.
