CAJ | 학술논문

建立了用 HPLC法测定番茄红素含量的分析方法，采用ODS色谱柱（150 mm ×4．6 mm ，5μm），流动相为乙腈‐甲醇‐二氯甲烷（60∶25∶15），检测波长472 nm ，柱温32℃。试验结果表明，番茄红素在0．2～40μg／mL范围内线性关系良好（ r＝0．9996），加标回收率大于96．0％，并可同时分离番茄红素和共存的β‐胡萝卜素。该方法准确、可靠，适用于番茄红素胶囊中番茄红素的含量测定。
건립료용 HPLC법측정번가홍소함량적분석방법，채용ODS색보주（150 mm ×4．6 mm ，5μm），류동상위을정‐갑순‐이록갑완（60∶25∶15），검측파장472 nm ，주온32℃。시험결과표명，번가홍소재0．2～40μg／mL범위내선성관계량호（ r＝0．9996），가표회수솔대우96．0％，병가동시분리번가홍소화공존적β‐호라복소。해방법준학、가고，괄용우번가홍소효낭중번가홍소적함량측정。
HPLC method was developed for the determination of lycopene by ODS column (150 mm × 4 .6 mm ,5μm) under the conditions as:acetonitrile‐methyl alcohol‐dichloromethane(60∶25∶15)as the mobile phase ,UV detection wavelength at 472 nm and column temperature at 32 ℃ .Experimental results showed that lycopene has a good linear relation over the range of 0 .2-40 μg/mL(r=0 .999 6)and the average recovery is over 96 .0 % .Lycopene andβ‐carotene are seperated well under the chromatographic conditions .The method proposed is accurate and reliable for determination of lycopene in lycopene capsule with satisfactory results .