CAJ | 학술논문

在对博落回防御素研究的基础上，于 NCBI 数据库中系统搜索罂粟科所有已登录的 EST 序列文库，推测出31条新颖的罂粟科植物防御素。分析了这些防御素的相对分子质量、pI 值、净电荷、疏水性平均值、蛋白结合能力、信号肽、亚细胞定位及其三级结构。
재대박락회방어소연구적기출상，우 NCBI 수거고중계통수색앵속과소유이등록적 EST 서렬문고，추측출31조신영적앵속과식물방어소。분석료저사방어소적상대분자질량、pI 치、정전하、소수성평균치、단백결합능력、신호태、아세포정위급기삼급결구。
Many researches focus on the medicinal value of alkaloids of Papaveraceae plants.However,the host non -spe-cific defense system,defensin,which contributes the broad -spectrum antimicrobial activity to the plants,are the same pre-cious for study.Based on the analysis of the cDNA clones of defensins we identified from Macleaya cordata,a species of Papaveraceae,31 novel defensins in Papaveraceae are identified by searching on the all registered Expressed Sequence Tags (ESTs)from the whole family Papaveraceae in the NCBI database.The molecular weight,pI value,net charge,the av-erage hydrophobicity,protein -binding Potential (Boman index),signal peptide,subcellular localization and tertiary struc-ture of the novel identified defensins are predicted and analyzed.The properties of the defensins from Papaveraceae are summarized,and the genetic evolution are analyzed.This study provides a new perspective to study the antibacterial sub-stances in Papaveraceae.