家畜生态学报
傢畜生態學報
가축생태학보
ACTA ECOLOGAE ANIMALIS DOMASTICI
2014年
10期
19-22
,共4页
赵恒鑫%何树芳%王嘉福%冉雪琴
趙恆鑫%何樹芳%王嘉福%冉雪琴
조항흠%하수방%왕가복%염설금
拷贝数变异%KIT基因%嗅觉受体%从江香猪
拷貝數變異%KIT基因%嗅覺受體%從江香豬
고패수변이%KIT기인%후각수체%종강향저
copy number variation%KIT gene%olfactory receptor%qPCR%Congjiang Xiang pig
为了研究从江香猪生长和繁殖差异与拷贝数变异拷贝数变异(copynumbervaria‐tion,CVN)之间的关系,采用实时荧光定量PCR方法,对从江香猪和大白猪基因组中的CNVR100与CNVR373的拷贝数进行了测定,并分析了从江香猪拷贝数与生长繁育性状之间的相关性。结果表明,从江香猪和大白猪基因组中都检测到CNVR100和CNVR373;与大白猪相比,从江香猪的CNVR100拷贝数较低,CNVR373拷贝数较高;相关性分析结果显示,从江香猪2个CNVRs与其体长和胸围有一定的负相关关系,表明这2个CNVRs的多态性可能对从江香猪的生长有一定的影响。
為瞭研究從江香豬生長和繁殖差異與拷貝數變異拷貝數變異(copynumbervaria‐tion,CVN)之間的關繫,採用實時熒光定量PCR方法,對從江香豬和大白豬基因組中的CNVR100與CNVR373的拷貝數進行瞭測定,併分析瞭從江香豬拷貝數與生長繁育性狀之間的相關性。結果錶明,從江香豬和大白豬基因組中都檢測到CNVR100和CNVR373;與大白豬相比,從江香豬的CNVR100拷貝數較低,CNVR373拷貝數較高;相關性分析結果顯示,從江香豬2箇CNVRs與其體長和胸圍有一定的負相關關繫,錶明這2箇CNVRs的多態性可能對從江香豬的生長有一定的影響。
위료연구종강향저생장화번식차이여고패수변이고패수변이(copynumbervaria‐tion,CVN)지간적관계,채용실시형광정량PCR방법,대종강향저화대백저기인조중적CNVR100여CNVR373적고패수진행료측정,병분석료종강향저고패수여생장번육성상지간적상관성。결과표명,종강향저화대백저기인조중도검측도CNVR100화CNVR373;여대백저상비,종강향저적CNVR100고패수교저,CNVR373고패수교고;상관성분석결과현시,종강향저2개CNVRs여기체장화흉위유일정적부상관관계,표명저2개CNVRs적다태성가능대종강향저적생장유일정적영향。
To investigate the polymorphism of copy number variation (CNV) and its relationship with growth and reproduction of Congjiang Xiang pig ,two CNVR numbers of Congjiang Xiang pig were deter‐mined by real-time quantitative polymerase chain reaction (qPCR) ,taking Yorkshire pig as control .The results showed that both of CNVR100 and CNVR373 were detected from the genome of Congjiang Xiang and Yorkshire pig breeds .The copy numbers of CNVR100 was lower and CNVR373 copies was higher in Congjiang Xiang pig than that in Yorkshire .Further more ,copies of two CNVRs were weakly negatively correlated with the value of body length and chest circumference of Congjiang Xiang pig .The results sug‐gested that both of two CNVs ,CNVR100 and CNVR373 ,might effect on the growth of body trunk and chest in Congjiang Xiang pig .