CAJ | 학술논문

利用三维荧光光谱结合交替归一加权残差算法（ANWE），对碳酸饮料中胭脂红含量的直接测定。首先通过使用英国爱丁堡公司生产的FLS920P荧光光谱仪测量所配制的胭脂红和日落黄混合溶液样品的三维荧光光谱，利用ANWE算法来进行解析，得到校正集中浓度与真实浓度的相关系数为0.9917，平均回收率为100.92％±2.71％，结果表明，ANWE算法可靠性比较好；然后把市售碳酸饮料稀释8，9，12，13倍，分别测量它们的三维荧光光谱，结合ANWE算法进行解析，计算得到校正集中浓度和实际浓度的相关系数分别为0.9930，0.9930，0.9932，0.9932，以及饮料中胭脂红含量分别为38.88，37.71，37.68和39.65μg?mL －1，平均浓度为（38.48±0.96）μg?mL －1；最后，为了验证所得饮料中胭脂红浓度的准确性，使用标准添加法，解析得到，校正样品中胭脂红的校正浓度和真实浓度相关系数为0.9935，且平均回收率为102.99％±2.15％。检测结果可为饮料中食品色素的快速定量提供一种新的思路。
이용삼유형광광보결합교체귀일가권잔차산법（ANWE），대탄산음료중연지홍함량적직접측정。수선통과사용영국애정보공사생산적FLS920P형광광보의측량소배제적연지홍화일락황혼합용액양품적삼유형광광보，이용ANWE산법래진행해석，득도교정집중농도여진실농도적상관계수위0.9917，평균회수솔위100.92％±2.71％，결과표명，ANWE산법가고성비교호；연후파시수탄산음료희석8，9，12，13배，분별측량타문적삼유형광광보，결합ANWE산법진행해석，계산득도교정집중농도화실제농도적상관계수분별위0.9930，0.9930，0.9932，0.9932，이급음료중연지홍함량분별위38.88，37.71，37.68화39.65μg?mL －1，평균농도위（38.48±0.96）μg?mL －1；최후，위료험증소득음료중연지홍농도적준학성，사용표준첨가법，해석득도，교정양품중연지홍적교정농도화진실농도상관계수위0.9935，차평균회수솔위102.99％±2.15％。검측결과가위음료중식품색소적쾌속정량제공일충신적사로。
Three-dimensional fluorescence spectra combined with second-order calibration algorithm based on alternate a weighted residual (ANWE) was applied to the direct concentration determination of carmine in carbonated beverages .Firstly ,3-D fluores-cence spectra of carmine and sunset yellow mixed solutions with different concentrations were obtained by employing spectrome-ter ,and analyzed by using ANWE ,the correlation coefficient between calibration concentration and the actual concentration was 0.991 7 ,and the average recovery was 100.92% ± 2.71% .The results show that the ANWE algorithm is reliable .Then ,the commercial carbonated soft drinks in 8 ,9 ,12 and 13 times diluted concentration were detected by using ANWE algorithm ,the correlation coefficient between relative concentration and the actual concentration were 0.993 0 ,0.993 0 ,0.993 2 and 0.793 2 , respectively ,and the contents of carmine in beverage were 38.88 ,37.71 ,37.68 and 39.65 μg?mL -1 ,respectively .The aver-age concentration was (38.48 ± 0.96)μg?mL -1 .Finally ,the standard addition method was applied to estimate the prediction accuracy between calibration concentration and the actual concentration was 0.993 5 ,and the average recovery was 102.99% ± 2.15% .The results can provide a new idea for the rapid content determination of food pigments in commercial beverages .