CAJ | 학술논문

蒜头果蛋白（malanin）是从云南、广西特有植物蒜头果中分离纯化出的一种新的、具有抗肿瘤活性的糖蛋白。用荧光光谱法研究了Cu2＋，Ag＋和Ca2＋对malanin和apo-malanin溶液（经EDTA透析脱去金属离子后的脱金属蛋白）荧光强度的影响。结果表明，Cu2＋对malanin和apo-malanin荧光均有明显的静态猝灭现象，其离解常数 Kd 分别为2.37×10－4和2.66×10－4 mol?L －1；Ag＋对malanin的荧光强度变化不大，但对apo-malanin荧光强度却有明显的静态猝灭现象，其离解常数 Kd 为2.37×10－4 mol? L －1；而Ca2＋对malanin和apo-malanin荧光强度均无明显变化，说明Ca2＋对维持malanin分子天然构象具有重要作用。
산두과단백（malanin）시종운남、엄서특유식물산두과중분리순화출적일충신적、구유항종류활성적당단백。용형광광보법연구료Cu2＋，Ag＋화Ca2＋대malanin화apo-malanin용액（경EDTA투석탈거금속리자후적탈금속단백）형광강도적영향。결과표명，Cu2＋대malanin화apo-malanin형광균유명현적정태졸멸현상，기리해상수 Kd 분별위2.37×10－4화2.66×10－4 mol?L －1；Ag＋대malanin적형광강도변화불대，단대apo-malanin형광강도각유명현적정태졸멸현상，기리해상수 Kd 위2.37×10－4 mol? L －1；이Ca2＋대malanin화apo-malanin형광강도균무명현변화，설명Ca2＋대유지malanin분자천연구상구유중요작용。
A novel protein with anti-tumor activities named malanin was isolated and purified from an endemic plant in Yunnan and Guangxi provinces .Effects of copper ion ,silver ion and calcium ion on malanin and apo-malanin fluorescence spectra were studied .The results showed that copper ion leads to obvious statistic quenching of malanin and apo-malanin fluorescence .The dissociation constant of them from malanin and apo-malanin were about 2.37 × 10-4 and 2.66 × 10-4 mol? L -1 ,respectively . The silver ion did not have quenching action on malanin fluorescence ,but it had statistic quenching effect on apo-malanin fluores-cence ,and its dissociation constant was 2.37 × 10-4 mol?L -1 .Calcium ion did not have quenching action on malanin and apo-malanin fluorescence .It plays an important role in keeping malanin natural conformation .