中华实验和临床感染病杂志（电子版）
中華實驗和臨床感染病雜誌（電子版）
중화실험화림상감염병잡지（전자판）
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL INFECTIOUS DISEASES(ELECTRONIC VERSION)
2014年
5期
683-686
,共4页
多重耐药%鲍曼不动杆菌%β-内酰胺类%耐药基因
多重耐藥%鮑曼不動桿菌%β-內酰胺類%耐藥基因
다중내약%포만불동간균%β-내선알류%내약기인
Multi-drug resistant%Acinetobacter baumannii%β-lactam%Drug resistance gene
目的：调查β-内酰胺类耐药基因在院内不同时间段分离的多重耐药鲍曼不动杆菌中的存在情况。方法采用PCR法检测β-内酰胺类相关耐药基因，并对部分阳性基因进行测序。结果2010年6月至2011年6月临床分离的46株多重耐药鲍曼不动杆菌中，其中41株（89.1%）含OXA23组基因、17株（37.0%）含PER基因、6株（13.0%）含IMP基因，6株（13.0%）膜孔蛋白基因carO缺失。2012年12月至2013年1月本院临床分离的42株多重耐药鲍曼不动杆菌中，41株（97.6%）含OXA23组基因，35株（83.3%）含TEM基因，42株（100%）含OXA64组基因和膜孔蛋白基因carO。经测序IMP阳性基因为IMP-4型金属酶基因，OXA23组阳性基因为OXA-23型碳青霉烯酶基因，OXA64组均为OXA-66型碳青霉烯酶基因，PER为PER-1型超广谱β-内酰胺酶基因、TEM为TEM-1型β-内酰胺酶基因。结论院内不同时间分离的多重耐药鲍曼不动杆菌OXA-23型碳青霉烯酶基因和膜孔蛋白基因carO检出率一直很高，IMP、PER、TEM和OXA64组β-内酰胺类耐药基因不同时间段检出情况差异很大。
目的：調查β-內酰胺類耐藥基因在院內不同時間段分離的多重耐藥鮑曼不動桿菌中的存在情況。方法採用PCR法檢測β-內酰胺類相關耐藥基因，併對部分暘性基因進行測序。結果2010年6月至2011年6月臨床分離的46株多重耐藥鮑曼不動桿菌中，其中41株（89.1%）含OXA23組基因、17株（37.0%）含PER基因、6株（13.0%）含IMP基因，6株（13.0%）膜孔蛋白基因carO缺失。2012年12月至2013年1月本院臨床分離的42株多重耐藥鮑曼不動桿菌中，41株（97.6%）含OXA23組基因，35株（83.3%）含TEM基因，42株（100%）含OXA64組基因和膜孔蛋白基因carO。經測序IMP暘性基因為IMP-4型金屬酶基因，OXA23組暘性基因為OXA-23型碳青黴烯酶基因，OXA64組均為OXA-66型碳青黴烯酶基因，PER為PER-1型超廣譜β-內酰胺酶基因、TEM為TEM-1型β-內酰胺酶基因。結論院內不同時間分離的多重耐藥鮑曼不動桿菌OXA-23型碳青黴烯酶基因和膜孔蛋白基因carO檢齣率一直很高，IMP、PER、TEM和OXA64組β-內酰胺類耐藥基因不同時間段檢齣情況差異很大。
목적：조사β-내선알류내약기인재원내불동시간단분리적다중내약포만불동간균중적존재정황。방법채용PCR법검측β-내선알류상관내약기인，병대부분양성기인진행측서。결과2010년6월지2011년6월림상분리적46주다중내약포만불동간균중，기중41주（89.1%）함OXA23조기인、17주（37.0%）함PER기인、6주（13.0%）함IMP기인，6주（13.0%）막공단백기인carO결실。2012년12월지2013년1월본원림상분리적42주다중내약포만불동간균중，41주（97.6%）함OXA23조기인，35주（83.3%）함TEM기인，42주（100%）함OXA64조기인화막공단백기인carO。경측서IMP양성기인위IMP-4형금속매기인，OXA23조양성기인위OXA-23형탄청매희매기인，OXA64조균위OXA-66형탄청매희매기인，PER위PER-1형초엄보β-내선알매기인、TEM위TEM-1형β-내선알매기인。결론원내불동시간분리적다중내약포만불동간균OXA-23형탄청매희매기인화막공단백기인carO검출솔일직흔고，IMP、PER、TEM화OXA64조β-내선알류내약기인불동시간단검출정황차이흔대。
Objective To investigate the prevalence of β-lactamase genes in multidrug-resistant Acinetobacter baumannii isolated in our hospital at different times. Methods The β-lactamase genes were analyzed by polymerase chain reaction (PCR), and part of the positive genes were veriifed by DNA sequencing. Results In the 46 multi-drug resistant Acinetobacter baumannii stains collected from June 2010 to June 2011, OXA23 group genes were found in 41 (89.1%) strains, PER in 17 (37.0%) stains;there were 6 (13.0%) stains carried IMP and loss of Porin gene (carO) was identiifed in 6 (13.0%) stains. In the 42 multi-drug resistant Acinetobacter baumannii stains collected from December 2012 to January 2013, OXA23 group genes was found in 41 (97.6%) strains, 35 (83.3%) stains carried TEM, OXA64 group genes and Porin gene (carO) was identiifed in 42 (100%) stains. By DNA sequencing, the positive genes IMP were veriifed as IMP-4 type metal enzyme gene, OXA23 group genes were OXA-23-type carbapenemase gene, OXA64 group genes were all OXA-66-type carbapenemase genes, PER was PER-1 type extended-spectrumβ-lactamase gene, and TEM was TEM-1-typeβ-lactamase gene. Conclusions The detection rates of the genes as OXA-23-type carbapenemase gene and Porin gene (carO) have been high in multi-drug resistant Acinetobacter baumannii isolated in hospital at different times, and detection rates ofβ-lactamase genes IMP, PER, TEM and OXA64 group varied greatly at different times.
